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首页> 外文期刊>Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines >Comparison of culture, polymerase chain reaction (PCR), TaqMan Salmonella, and Transia Card Salmonella assays for detection of Salmonella spp. in naturally-contaminated ground chicken, ground turkey, and ground beef.
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Comparison of culture, polymerase chain reaction (PCR), TaqMan Salmonella, and Transia Card Salmonella assays for detection of Salmonella spp. in naturally-contaminated ground chicken, ground turkey, and ground beef.

机译：用于检测沙门氏菌的培养物，聚合酶链反应（PCR），TaqMan沙门氏菌和Transia Card沙门氏菌检测方法的比较。在自然污染的碎鸡肉，碎火鸡和碎牛肉中食用。

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Four types of assays were evaluated for the detection of Salmonella spp. in retail ground chicken (86 packages), ground turkey (104 packages), and ground beef (54 packages). Two 25 g samples from each package were separately subjected to pre-enrichment in buffered peptone water for 20h at 37 degrees C followed by enrichment in Rappaport Vassiliadis (RV) broth for 20 h at 42 degrees C. The RV enrichments were plated onto Rambach agar, Rainbow Agar Salmonella, and XLT4 agar, and were also tested by a PCR assay targeting the Salmonella invA gene, as well as by the TaqMan((R)) Salmonella PCR assay. Additionally, the RV enrichments were tested using the Transia Card Salmonella((R)) immunoassay. Results showed that 16.8, 24.0, 28.8, and 26.4% of turkey samples were positive for Salmonella spp. by culture, PCR, TaqMan PCR, and Transia Card Salmonella assays, respectively. Eighteen, 28.5, 35.5, and 34.9% of chicken samples were positive by culture, PCR, TaqMan PCR, and Transia Card Salmonella assays, respectively, and 6.5, 6.5, 6.5, and 18.5% of ground beef samples were positive by the four assays, respectively. Analysis of the data using the kappa statistic showed that there was substantial to excellent agreement between the PCR and TaqMan PCR assays and between the PCR and culture assays (kappa coefficients ranging from 0.67 to 0.87), while there was poor to fair agreement between the results of the Transia Card Salmonella assay and the other methods (kappa coefficients ranging from 0.28 to 0.32). Overall, results showed that the PCR-based assays were more sensitive than the culture method, and the culture and PCR-based assays were more specific than the immunoassay for detection of Salmonella in ground chicken, turkey, and beef due to the occurrence of false positive results using the immunoassay.
机译：评估了四种类型的测定方法以检测沙门氏菌。零售碎鸡肉（86包），火鸡（104包）和碎牛肉（54包）。将来自每个包装的两个25 g样品分别在37℃的蛋白p缓冲液中预富集20 h，然后在42℃的Rappaport Vassiliadis（RV）肉汤中富集20 h。将RV富集接种到Rambach琼脂上，Rainbow Agar沙门氏菌和XLT4琼脂，也通过针对沙门氏菌invA基因的PCR检测法和TaqMan沙门氏菌PCR检测法进行了测试。另外，使用Transia CardSalmonella免疫测定法测试RV富集。结果显示，分别有16.8％，24.0％，28.8％和26.4％的火鸡样品沙门氏菌呈阳性。分别通过培养，PCR，TaqMan PCR和Transia Card Salmonella分析。分别通过培养，PCR，TaqMan PCR和Transia Card Salmonella分析呈阳性的鸡肉样本分别为18％，28.5％，35.5％和34.9％，而通过四种分析呈阳性的鸡肉样本分别为6.5％，6.5％，6.5％，6.5％和18.5％。 ， 分别。使用kappa统计量进行的数据分析显示，PCR和TaqMan PCR分析之间以及PCR和培养分析之间存在着非常良好的一致性（kappa系数介于0.67至0.87之间），而结果之间的一致性差强人意Transia Card沙门氏菌测定法和其他方法（卡帕系数介于0.28至0.32之间）。总体而言，结果表明，基于PCR的检测方法比培养方法更灵敏，并且由于发生了假的，基于培养物和基于PCR的检测方法比免疫检测方法更特异性地检测沙门氏菌，火鸡和牛肉中的沙门氏菌使用免疫测定获得阳性结果。

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