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首页> 外文期刊>Molecular and Cellular Biochemistry: An International Journal for Chemical Biology >Modulation of Sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes.
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Modulation of Sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes.

机译：白藜芦醇和烟酰胺对Sirt1的调节改变了猪前脂肪细胞的增殖和分化。

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摘要

Sirt1, a NAD(+)-dependent histone deacetylase, may regulate senescence, metabolism, and apoptosis. In this study, primary pig preadipocytes were cultured in DMEM/F12 medium containing 10% fetal bovine serum (FBS) with or without reagents affecting Sirt1 activity. The adipocyte differentiation process was visualized by light microscopy after Oil red O staining. Proliferation and differentiation of preadipocytes was measured using methylthiazolyldiphenyl-tetrazolium bromide (MTT) and Oil red O extraction. Expression of Sirt1, FoxO1, and adipocyte specific genes was detected with semi-quantitive RT-PCR. The results showed that Sirt1 mRNA was widely expressed in various pig tissues from different developmental stages. Sirt1 mRNA was expressed throughout the entire differentiation process of pig preadipocytes. Resveratrol significantly increased Sirt1 mRNA expression, but decreased the expression of FoxO1 and adipocyte marker gene PPARgamma2. Resveratrol significantly inhibited pig preadipocyte proliferation and differentiation. Nicotinamide decreased the expression of Sirt1 mRNA, but increased the expression of FoxO1 and adipocyte specific genes. Nicotinamide greatly stimulated the proliferation and differentiation of pig preadipocytes. In conclusion, these results indicate that Sirt1 may modulate the proliferation and differentiation of pig preadipocytes. Sirt1 may down-regulate pig preadipocytes proliferation and differentiation through repression of adipocyte genes or FoxO1.
机译：Sirt1，NAD（+）依赖的组蛋白脱乙酰基酶，可能调节衰老，代谢和凋亡。在这项研究中，在含有或不含影响Sirt1活性的试剂的10％胎牛血清（FBS）的DMEM / F12培养基中培养原代猪前脂肪细胞。油红O染色后，通过光学显微镜观察脂肪细胞的分化过程。使用甲基噻唑基二苯基溴化四唑（MTT）和油红O提取液测量前脂肪细胞的增殖和分化。用半定量RT-PCR检测Sirt1，FoxO1和脂肪细胞特异性基因的表达。结果表明，Sirt1 mRNA在不同发育阶段的各种猪组织中广泛表达。 Sirt1 mRNA在猪前脂肪细胞的整个分化过程中表达。白藜芦醇显着增加Sirt1 mRNA表达，但降低FoxO1和脂肪细胞标记基因PPARgamma2的表达。白藜芦醇显着抑制猪前脂肪细胞的增殖和分化。烟酰胺降低Sirt1 mRNA的表达，但增加FoxO1和脂肪细胞特异性基因的表达。烟酰胺极大地刺激了猪前脂肪细胞的增殖和分化。总之，这些结果表明Sirt1可能调节猪前脂肪细胞的增殖和分化。 Sirt1可能通过抑制脂肪细胞基因或FoxO1来下调猪前脂肪细胞的增殖和分化。

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