...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Solution structure of the sodium channel inactivation gate.
【24h】

Solution structure of the sodium channel inactivation gate.

机译：钠通道失活门的溶液结构。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The sodium channel initiates action potentials by opening in response to membrane depolarization. Fast channel inactivation, which is required for proper physiological function, is mediated by a cytoplasmic loop proposed to occlude the ion pore via a hinged lid mechanism with the triad IFM serving as a hydrophobic "latch". The NMR solution structure of the isolated inactivation gate reveals a stably folded core comprised of an alpha-helix capped by an N-terminal turn, supporting a model in which the tightly folded core containing the latch motif pivots on a more flexible hinge region to occlude the pore during inactivation. The structure, in combination with substituted cysteine mutagenesis experiments, indicates that the IFM triad and adjacent Thr are essential components of the latch and suggests differing roles for the residues of the IFMT motif in fast inactivation.
机译：钠通道通过响应膜去极化而打开来启动动作电位。快速通道的失活是适当的生理功能所必需的，其由胞质环介导的，该胞质环被提议通过铰链盖机制将离子孔闭塞，而三元组IFM用作疏水性“闩锁”。分离的灭活门的NMR溶液结构揭示了一个稳定折叠的核，该核由被N端匝覆盖的α螺旋组成，支持其中包含闩锁基序的紧密折叠的核在一个更灵活的铰链区域上枢转以闭塞的模型灭活过程中的毛孔。该结构与取代的半胱氨酸诱变实验相结合，表明IFM三联体和相邻的Thr是闩锁的重要组成部分，并暗示了IFMT基序残基在快速失活中的不同作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号