• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular biology of the cell >Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo
【24h】

Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo

机译：秀丽隐杆线虫核孔蛋白Nup93和Nup205确定体内核孔复合体大小排阻的极限

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nuclear pore complexes (NPCs) span the nuclear envelope and mediate communication between the nucleus and the cytoplasm. To obtain insight into the structure and function of NPCs of multicellular organisms, we have initiated an extensive analysis of Caenorhabditis elegans nucleoporins. Of 20 assigned C. elegans nucleoporin genes, 17 were found to be essential for embryonic development either alone or in combination. In several cases, depletion of nucleoporins by RNAi caused severe defects in nuclear appearance. More specifically, the C. elegans homologs of vertebrate Nup93 and Nup205 were each found to be required for normal NPC distribution in the nuclear envelope in vivo. Depletion of Nup93 or Nup205 caused a failure in nuclear exclusion of nonnuclear macromolecules of similar to70 kDa without preventing active nuclear protein import or the assembly of the nuclear envelope. The defects in NPC exclusion were accompanied by abnormal chromatin condensation and early embryonic arrest. Thus, the contribution to NPC structure of Nup93 and Nup205 is essential for establishment of normal NPC function and for cell viability. [References: 49]
机译：核孔复合物（NPC）跨越核被膜，并介导核与细胞质之间的通讯。为了深入了解多细胞生物的NPC的结构和功能，我们已经开始对秀丽隐杆线虫核孔蛋白进行广泛的分析。在20个分配的秀丽隐杆线虫核孔蛋白基因中，有17个单独或组合对胚胎发育至关重要。在某些情况下，RNAi耗尽核孔蛋白会导致核外观出现严重缺陷。更具体地，发现脊椎动物Nup93和Nup205的秀丽隐杆线虫同源物各自是体内核膜中正常NPC分布所必需的。 Nup93或Nup205的耗尽导致类似70 kDa的非核大分子的核排斥失败，而没有阻止活性核蛋白的导入或核膜的组装。 NPC排斥的缺陷伴有染色质异常凝集和早期胚胎停滞。因此，对Nup93和Nup205的NPC结构的贡献对于建立正常的NPC功能和细胞活力至关重要。 [参考：49]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号