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首页> 外文期刊>Molecular biology of the cell >Ch14p and Im13p are two new members of the budding yeast outer kinetochore
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Ch14p and Im13p are two new members of the budding yeast outer kinetochore

机译：Ch14p和Im13p是发芽酵母外生线粒体的两个新成员

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摘要

Kinetochore proteins contribute to the fidelity of chromosome transmission by mediating the attachment of a specialized chromosomal region, the centromere, to the mitotic spindle during mitosis. In budding yeast, a subset of kinetochore proteins, referred to as the outer kinetochore, provides a link between centromere DNA-binding proteins of the inner kinetochore and microtubule-binding proteins. Using a combination of chromatin immunoprecipitation, in vivo localization, and protein coimmunoprecipitation, we have established that yeast Chl4p and Iml3p are outer kinetochore proteins that localize to the kinetochore in a Ctf19p-dependent manner. Chl4p interacts with the outer kinetochore proteins Ctf19p and Ctf3p, and Iml3p interacts with Chl4p and Ctf19p. In addition, Chl4p is required for the Ctf19p-Ctf3p and Ctf19p-Iml3p interactions, indicating that Chl4p is an important structural component of the outer kinetochore. These physical interaction dependencies provide insights into the molecular architecture and centromere DNA loading requirements of the outer kinetochore complex. [References: 48]
机译：线粒体蛋白通过介导有丝分裂过程中特定的染色体区域着丝粒附着于有丝分裂纺锤体，从而促进了染色体传输的保真度。在发芽酵母中，线粒体蛋白的一个子集（称为外部线粒体）提供了内部线粒体着丝粒DNA结合蛋白与微管结合蛋白之间的联系。使用染色质免疫沉淀，体内定位和蛋白质共免疫沉淀的组合，我们已经建立了酵母Chl4p和Iml3p是外部线粒体蛋白，其以Ctf19p依赖性的方式定位于线粒体。 Chl4p与外部动粒蛋白Ctf19p和Ctf3p相互作用，而Iml3p与Chl4p和Ctf19p相互作用。此外，Ctl4p是Ctf19p-Ctf3p和Ctf19p-Iml3p相互作用所必需的，表明Chl4p是外部动粒的重要结构成分。这些物理相互作用的依赖性提供了对外部动粒复合物的分子结构和着丝粒DNA负载要求的见解。 [参考：48]

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