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首页> 外文期刊>Molecular biology of the cell >Distinct in vivo dynamics of vertebrate SUMO paralogues
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Distinct in vivo dynamics of vertebrate SUMO paralogues

机译：脊椎动物SUMO旁系同源动物的不同体内动力学

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摘要

There are three mammalian SUMO paralogues: SUMO-1 is similar to 45% identical to SUMO-2 and SUMO-3, which are 96% identical to each other. It is currently unclear whether SUMO-1, -2, and -3 function in ways that are unique, redundant, or antagonistic. To address this question, we examined the dynamics of individual SUMO paralogues by using cell lines that stably express each of the mammalian SUMO proteins fused to the yellow fluorescent protein (YFP). Whereas SUMO-2 and -3 showed very similar distributions throughout the nucleoplasm, SUMO-1 was uniquely distributed to the nuclear envelope and to the nucleolus. Photobleaching experiments revealed that SUMO-1 dynamics was much slower than SUMO-2 and -3 dynamics. Additionally, the mobility of SUMO paralogues differed between subnuclear structures. Finally, the timing and distributions were dissimilar between paralogues as cells exited from mitosis. SUMO-1 was recruited to nuclear membrane as nuclear envelopes reformed in late anaphase, and accumulated rapidly into the nucleus. SUMO-2 and SUMO-3 localized to chromosome earlier and accumulated gradually during telophase. Together, these findings demonstrate that mammalian SUMO-1 shows patterns of utilization that are clearly discrete from the patterns of SUMO-2 and -3 throughout the cell cycle, arguing that it is functionally distinct and specifically regulated in vivo.
机译：共有三种哺乳动物SUMO旁系同源物：SUMO-1与SUMO-2和SUMO-3的相似性为45％，彼此之间的同源性为96％。目前尚不清楚SUMO-1，-2和-3是否以独特，冗余或拮抗的方式起作用。为了解决这个问题，我们通过使用稳定表达融合到黄色荧光蛋白（YFP）的每个哺乳动物SUMO蛋白的细胞系来检查单个SUMO旁系同源物的动力学。 SUMO-2和-3在整个核质中显示出非常相似的分布，而SUMO-1则独特地分布在核膜和核仁中。光漂白实验表明，SUMO-1动力学比SUMO-2和-3动力学慢得多。此外，SUMO旁系同源物的迁移性在亚核结构之间有所不同。最后，当细胞从有丝分裂中退出时，旁系同源物的时间和分布是不同的。 SUMO-1被募集到核膜，因为在后期后期核膜被改造，并迅速积累到核中。 SUMO-2和SUMO-3较早定位于染色体，并在末期逐渐积累。在一起，这些发现表明哺乳动物SUMO-1的利用模式明显不同于整个细胞周期中SUMO-2和-3的模式，认为它在功能上是不同的，并且在体内受到特定调节。

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