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首页> 外文期刊>Molecular biology of the cell >IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending
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IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending

机译：IC138是WD重复的动力蛋白中间链，用于轻链组装和调节鞭毛弯曲

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Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation controls flagellar bending, we cloned the IC138 gene. IC138 encodes a novel protein with a calculated mass of 111 kDa and is predicted to form seven WD-repeats at the C terminus. IC138 maps near the BOP5 locus, and bop5-1 contains a point mutation resulting in a truncated IC138 lacking the C terminus, including the seventh WD-repeat. bop5-1 cells display wild-type flagellar beat frequency but swim slower than wild-type cells, suggesting that bop5-1. is altered in its ability to control flagellar waveform. Swimming speed is rescued in bop5-1 transformants containing the wild-type 1038, confirming that BOP5 encodes IC138. With the exception of the roadblock-related light chain, LC7b, all the other known components of the 11 complex, including the truncated IC138, are assembled in bop5-1 axonemes. Thus, the bop5-1 motility phenotype reveals a role for IC138 and LC7b in the control of flagellar bending. IC138 is hyperphosphorylated in paralyzed flagellar mutants lacking radial spoke and central pair components, further indicating a role for the radial spokes and central pair apparatus in control of IC138 phosphorylation and regulation of flagellar waveform.
机译：动力蛋白IC IC138磷酸化的增加与鞭毛微管滑动和趋光性缺陷的减少有关。为了检验IC138磷酸化调控控制鞭毛弯曲的假说，我们克隆了IC138基因。 IC138编码一种新蛋白质，计算质量为111 kDa，并预计在C末端形成七个WD重复序列。 IC138定位在BOP5基因座附近，并且bop5-1包含一个点突变，导致缺少C末端（包括第七个WD重复序列）的IC138被截短。 bop5-1细胞显示野生型鞭毛搏动频率，但游动速度慢于野生型细胞，表明bop5-1。改变了鞭毛波形的控制能力。在包含野生型1038的bop5-1转化子中挽救了游泳速度，证实BOP5编码IC138。除了与路障相关的轻链LC7b以外，这11个联合体的所有其他已知组件（包括截短的IC138）都组装在bop5-1轴突中。因此，bop5-1运动表型揭示了IC138和LC7b在控制鞭毛弯曲中的作用。 IC138在缺乏放射状辐条和中央对组件的瘫痪鞭毛突变体中被高磷酸化，进一步表明放射状辐条和中央对设备在控制IC138磷酸化和调节鞭毛波形中的作用。

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