...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular biology of the cell >RNA polymerase III transcripts and the PTB protein are essential for the integrity of the perinucleolar compartment
【24h】

RNA polymerase III transcripts and the PTB protein are essential for the integrity of the perinucleolar compartment

机译：RNA聚合酶III转录本和PTB蛋白对于核周腔室的完整性至关重要

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The perinucleolar compartment (PNC) is a nuclear substructure present in transformed cells. The PNC is defined by high concentrations of certain RNA binding proteins and a subset of small RNAs transcribed by RNA polymerase III (pol III), including the signal recognition particle RNA and an Alu. RNA as reported here. To determine if the PNC is dependent on pol III transcription, HeLa cells were microinjected with the selective pol III inhibitor, Tagetin. This resulted in disassembly of the PNC, whereas inhibition of pol I by cycloheximide or pol II by a-amanitin did not significantly affect the PNC. However, overexpression of one of the PNC-associated RNAs from a pol II promoter followed by injection of Tagetin blocked the Tagetin-induced PNC disassembly, demonstrating that it is the RNA rather than pol III activity that is important for the PNC integrity. To elucidate the role of the PNC-associated protein PTB, its synthesis was inhibited by siRNA. This resulted in a reduction of the number of PNC-containing cells and the PNC size. Together, these findings suggest, as a working model, that PNCs may be involved in the metabolism of specific pol III transcripts in the transformed state and that PTB is one of the key elements mediating this process. [References: 33]
机译：周核室（PNC）是存在于转化细胞中的核亚结构。 PNC由高浓度的某些RNA结合蛋白和由RNA聚合酶III（pol III）转录的小RNA的子集定义，包括信号识别颗粒RNA和Alu。如此处报道的RNA。为了确定PNC是否依赖于pol III转录，将选择性pol III抑制剂Tagetin微注射入HeLa细胞。这导致PNC的拆卸，而环己酰亚胺对pol I的抑制或a- amanitin对pol II的抑制并没有显着影响PNC。但是，从pol II启动子中过表达一种PNC相关的RNA，然后注射他汀类药物会阻断他汀诱导的PNC的分解，这表明RNA对PNC完整性很重要，而不是pol III活性。为了阐明PNC相关蛋白PTB的作用，其合成受到siRNA的抑制。这导致包含PNC的小区数量和PNC大小的减少。总之，这些发现表明，作为一种工作模型，PNC可能参与转化状态下特定pol III转录物的代谢，而PTB是介导这一过程的关键因素之一。 [参考：33]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号