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首页> 外文期刊>Molecular biology of the cell >Fission yeast cdc31p is a component of the half-bridge and controls SPB duplication
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Fission yeast cdc31p is a component of the half-bridge and controls SPB duplication

机译：裂变酵母cdc31p是半桥的组成部分，可控制SPB复制

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The fission yeast spindle pole body (SPB) is a nucleus-associated organelle that duplicates once each cell cycle during interphase. Duplicated SPBs serve as the poles of an intranuclear mitotic spindle after their insertion into the nuclear envelope in mitosis (Ding et al., Mol. Biol. Cell 8, 1461-1479). Here, we report the identification and characterization of Schizosaccharomyces pombe cdc31p, a member of the conserved calcium-binding centrin/CDC31 family. Immunofluorescence and immunoelectron microscopy show that cdc31p is a SPB component localized at the half-bridge structure of the SPB. edc31 is an essential gene and Deltacdc31 cells and cdc31 conditional mutant cells arrest in mitosis with a monopolar mitotic spindle organized from a single SPB. EM analysis demonstrates that mutant cdc31 cells fail to duplicate the SPB. In addition, cdc31p exhibits genetic interactions with the SPB component sad1p and is required for sad1p localization. Finally, cdc31 mutant can undergo single or multiple rounds of septation before the exit from mitosis, suggesting that cdc31p activity or SPB duplication may be required for the proper coordination between the exit from mitosis and the initiation of septation. [References: 53]
机译：裂变酵母纺锤体极体（SPB）是一个与核相关的细胞器，在相间每个细胞周期复制一次。重复的SPB在插入有丝分裂的核膜中后充当核内有丝分裂纺锤体的极（Ding等人，分子生物学细胞，第8期，1461-1479）。在这里，我们报告鉴定和表征粟酒裂殖酵母cdc31p，保守的钙结合性centrin / CDC31家族的成员。免疫荧光和免疫电子显微镜检查显示，cdc31p是位于SPB半桥结构上的SPB组件。 edc31是必不可少的基因，Deltacdc31细胞和cdc31条件突变细胞在有丝分裂中停滞，并具有由单个SPB组成的单极有丝分裂纺锤体。 EM分析表明，突变的cdc31细胞无法复制SPB。另外，cdc31p与SPB组件sad1p表现出遗传相互作用，并且是sad1p定位所必需的。最后，cdc31突变体可以在有丝分裂退出之前经历单轮或多轮分离，这表明从有丝分裂退出和分离开始之间可能需要适当的cdc31p活性或SPB复制。 [参考：53]

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