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首页> 外文期刊>Molecular biology of the cell >She4p/Dim1p interacts with the motor domain of unconventional Myosins in the budding yeast, Saccharomyces cerevisiae
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She4p/Dim1p interacts with the motor domain of unconventional Myosins in the budding yeast, Saccharomyces cerevisiae

机译：She4p / Dim1p与发芽酵母酿酒酵母中非常规肌球蛋白的运动域相互作用

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摘要

She4p/Dim1p, a member of the UNC-45/CRO1/She4p (UCS) domain-containing protein family, is required for endocytosis, polarization of actin cytoskeleton, and polarization of ASH1 mRNA in Saccharomyces cerevisiae. We show herein that She4p/Dim1p is involved in endocytosis and actin polarization through interactions with the type I myosins Myo3p and Myo5p. Two-hybrid and biochemical experiments showed that She4p/Dim1p interacts with the motor domain of Myo3/5p through its UCS domain. She4p/Dim1p was required for Myo5p localization to cortical patch-like structures. Using random mutagenesis of the motor region of MYO5, we identified four independent dominant point mutations that suppress the temperature-sensitive growth phenotype of the she4/dim1 null mutant. All of the amino acid substitutions caused by these mutations, V164I, N168I, N209S, and K377M, could suppress the defects of endocytosis and actin polarization of the she4/dim1 mutant as well. She4p/Dim1p also showed two-hybrid interactions with the motor domain of a type H myosin Myo1p and type V myosins Myo2p and Myo4p, and was required for proper localization of Myo4p, which regulates polarization of ASH1 mRNA. Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins. [References: 66]
机译：She4p / Dim1p是包含UNC-45 / CRO1 / She4p（UCS）域的蛋白质家族的成员，对于酿酒酵母中的内吞作用，肌动蛋白细胞骨架极化和ASH1 mRNA极化是必需的。我们在这里显示，She4p / Dim1p通过与I型肌球蛋白Myo3p和Myo5p的相互作用参与了内吞作用和肌动蛋白极化。两次杂交和生化实验表明，She4p / Dim1p通过其UCS域与Myo3 / 5p的运动域相互作用。 She4p / Dim1p是Myo5p定位到皮质斑块状结构所必需的。使用随机诱变的MYO5的电机区域，我们确定了四个独立的优势点突变，这些突变抑制了she4 / dim1 null突变体的温度敏感型生长表型。由这些突变引起的所有氨基酸取代，V164I，N168I，N209S和K377M，也可以抑制she4 / dim1突变体的内吞作用和肌动蛋白极化缺陷。 She4p / Dim1p还显示出与H型肌球蛋白Myo1p和V型肌球蛋白Myo2p和Myo4p的运动域的两个杂交相互作用，并且是Myo4p正确定位（调节ASH1 mRNA极化）所必需的。我们的结果表明，She4p / Dim1p是结构完整性或非常规肌球蛋白运动域调节所必需的。 [参考：66]

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