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首页> 外文期刊>Molecular biology of the cell >Myo6 facilitates the translocation of endocytic vesicles from cell peripheries
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Myo6 facilitates the translocation of endocytic vesicles from cell peripheries

机译：Myo6促进细胞周围细胞内吞囊泡的移位

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Immunolocalization studies in epithelial cells revealed myo6 was associated with peripherally located vesicles that contained the transferrin receptor. Pulse-chase experiments after transferrin uptake showed that these vesicles were newly uncoated endocytic vesicles and that myo6 was recruited to these vesicles immediately after uncoating. GIPC, a putative myo6 tail binding protein, was also present. Myo6 was not present on early endosomes, suggesting that myo6 has a transient association with endocytic vesicles and is released upon early endosome fusion. Green fluorescent protein (GFP) fused to myo6 as well as the cargo-binding tail (M6tail) alone targeted to the nascent endocytic vesicles. Overexpression of GFP-M6tail had no effect on a variety of organelle markers; however, GFP-M6tail displaced the endogenous myo6 from nascent vesicles and resulted in a significant delay in transferrin uptake. Pulse-chase experiments revealed that transferrin accumulated in uncoated vesicles within the peripheries of transfected cells and that Rab5 was recruited to the surface of these vesicles. Given sufficient time, the transferrin did traffic to the perinuclear sorting endosome. These data suggest that myo6 is an accessory protein required for the efficient transportation of nascent endocytic vesicles from the actin-rich peripheries of epithelial cells, allowing for timely fusion of endocytic vesicles with the early endosome. [References: 43]
机译：在上皮细胞中的免疫定位研究表明myo6与周围含有转铁蛋白受体的囊泡有关。摄取转铁蛋白后的脉冲追踪实验表明，这些囊泡是新近未包被的内吞囊泡，并且在解包后立即将myo6募集到这些囊泡中。 GIPC，一种假定的myo6尾部结合蛋白，也存在。 Myo6不存在于早期的内体中，这表明myo6与内吞小泡具有短暂的关联，并在早期的内体融合时释放。与myo6融合的绿色荧光蛋白（GFP）以及仅针对新生内吞囊泡的载货结合尾巴（M6tail）。 GFP-M6tail的过表达对多种细胞器标记物没有影响。然而，GFP-M6tail取代了新生囊泡中的内源性myo6，并导致转铁蛋白的吸收显着延迟。脉冲追踪实验显示转铁蛋白积累在转染细胞周围未包被的囊泡中，Rab5被募集到这些囊泡的表面。给定足够的时间，转铁蛋白确实运输到核周分选内体。这些数据表明，myo6是从富含肌动蛋白的上皮细胞周边有效运输新生内吞囊泡所需的辅助蛋白，从而可以使内吞囊泡与早期内体及时融合。 [参考：43]

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