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首页> 外文期刊>Molecular biology of the cell >Keratin 20 helps maintain intermediate filament organization in intestinal epithelia
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Keratin 20 helps maintain intermediate filament organization in intestinal epithelia

机译：角蛋白20帮助维持肠上皮细胞的中间丝组织

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摘要

Of the >20 epithelial keratins, keratin 20 (K20) has an unusual distribution and is poorly studied. We began to address K20 function, by expressing human wild-type and Arg80-->His (R80H) genomic (18 kb) and cDNA K20 in cells and mice. Arg80 of K20 is conserved in most keratins, and its mutation in epidermal keratins causes several skin diseases. R80H but not wild-type K20 generates disrupted keratin filaments in transfected cells. Transgenic mice that overexpress K20 R80H have collapsed filaments in small intestinal villus regions, when expressed at moderate levels, whereas wild-type K20-overexpressing mice have normal keratin networks. Overexpressed K20 maintains its normal distribution in several tissues, but not in the pancreas and stomach, without causing any tissue abnormalities. Hence, K20 pancreatic and gastric expression is regulated outside the 18-kb region. Cross-breeding of wild-type or R80H K20 mice with mice that overexpress wild-type K18 or K18 that is mutated at the conserved K20 Arg80-equivalent residue show that K20 plays an additive and compensatory role with K18 in maintaining keratin filament organization in the intestine. Our data suggest, the presence of unique regulatory domains for pancreatic and gastric K20 expression and support a significant role for K20 in maintaining keratin filaments in intestinal epithelia. [References: 37]
机译：在20多种上皮角蛋白中，角蛋白20（K20）具有不寻常的分布，并且研究较少。我们开始通过在细胞和小鼠中表达人类野生型和Arg80-> His（R80H）基因组（18 kb）和cDNA K20来解决K20的功能。 K20的Arg80在大多数角蛋白中都是保守的，其在表皮角蛋白中的突变会引起多种皮肤疾病。 R80H但不是野生型K20会在转染的细胞中产生破裂的角蛋白丝。当以中等水平表达时，过表达K20 R80H的转基因小鼠的小肠绒毛区域的细丝塌陷，而过表达K20的野生型小鼠具有正常的角蛋白网络。过表达的K20在数个组织中均保持其正常分布，但在胰腺和胃中则不然，而不会引起任何组织异常。因此，K20胰腺和胃的表达在18 kb区域之外受到调控。将野生型或R80H K20小鼠与过量表达野生型K18或在保守的K20 Arg80等效残基处突变的K18的小鼠进行杂交繁殖，表明K20与K18在维持角蛋白丝组织中的功能上起着加和补偿作用。肠。我们的数据表明，胰腺和胃K20表达的独特调控域的存在，并支持K20在维持肠上皮中角蛋白丝的重要作用。 [参考：37]

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