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首页> 外文期刊>Molecular biology of the cell >Repeated cycles of rapid actin assembly and disassembly on epithelial cell phagosomes
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Repeated cycles of rapid actin assembly and disassembly on epithelial cell phagosomes

机译：肌动蛋白在上皮细胞吞噬体上快速组装和拆卸的重复周期

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摘要

We have found that early in infection of the intracellular pathogen Listeria monocytogenes in Madin-Darby canine kidney epithelial cells expressing actin conjugated to green fluorescent protein, F-actin rapidly assembles (similar to25 s) and disassembles (similar to30 s) around the bacteria, a phenomenon we call flashing. L. monocytogenes strains unable to perform actin-based motility or unable to escape the phagosome were capable of flashing, suggesting that the actin assembly occurs on the phagosome membrane. Cycles of actin assembly and disassembly could occur repeatedly on the same phagosome. Indirect immunofluorescence showed that most bacteria were fully internalized when flashing occurred, suggesting that actin flashing does not represent phagocytosis. Escherichia coli expressing invA, a gene product from Yersinia pseudotuberculosis that mediates cellular invasion, also induced flashing. Furthermore, polystyrene beads coated with E-cadherin or transferrin also induced flashing after internalization. This suggests that flashing occurs downstream of several distinct molecular entry mechanisms and may be a general consequence of internalization of large objects by epithelial cells.
机译：我们发现，在Madin-Darby犬肾上皮细胞中，表达与绿色荧光蛋白结合的肌动蛋白的细胞内病原体单核细胞增生性李斯特菌感染，F-肌动蛋白在细菌周围迅速组装（约25 s）并分解（约30 s），我们称之为闪烁的现象。单核细胞增生李斯特氏菌不能执行基于肌动蛋白的运动或不能逃脱吞噬体的能力能够闪烁，表明肌动蛋白组装发生在吞噬体膜上。肌动蛋白组装和拆卸的循环可以在同一吞噬体上重复发生。间接免疫荧光显示，当发生闪光时，大多数细菌已完全内在化，表明肌动蛋白闪光不代表吞噬作用。表达invA（介导细胞侵袭的假性耶尔森氏菌的基因产物）的大肠杆菌也诱导闪烁。此外，内在化后，涂有E-钙粘蛋白或运铁蛋白的聚苯乙烯珠粒也会引起闪光。这表明闪烁发生在几种不同的分子进入机制的下游，并且可能是上皮细胞内化大物体的一般结果。

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