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首页> 外文期刊>Molecular biology of the cell >Oxidative stress activates FUS1 and RLM1 transcription in the yeast Saccharomyces cerevisiae in an oxidant-dependent manner
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Oxidative stress activates FUS1 and RLM1 transcription in the yeast Saccharomyces cerevisiae in an oxidant-dependent manner

机译：氧化应激以依赖于氧化剂的方式激活酵母酿酒酵母中的FUS1和RLM1转录

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摘要

Mating in haploid Saccharomyces cerevisiae occurs after activation of the pheromone response pathway. Biochemical components of this pathway are involved in other yeast signal transduction networks. To understand more about the coordination between signaling pathways, we used a "chemical genetic" approach, searching for compounds that would activate the pheromone-responsive gene FUS1 and RLM1, a reporter for the cell integrity pathway. We found that catecholamines (L-3,4-hydroxyphenylalanine [L-dopa], dopamine, adrenaline, and noradrenaline) elevate FUS1 and RLM1 transcription. N-Acetyl-cysteine, a powerful antioxidant in yeast, completely reversed this effect, suggesting that FUS1 and RLM1 activation in response to catecholamines is a result of oxidative stress. The oxidant hydrogen peroxide also was found to activate transcription of an RLM1 reporter. Further genetic analysis combined with immunoblotting revealed that Kss1, one of the mating mitogen-activated protein kinases (MAPKs), and Mpk1., an MAPK of the cell integrity pathway, participated in L-dopa-induced stimulation of FUS1 and RLM1 transcription. We also report that Mpk1 and Hog1, the high osmolarity MAPK, were phosphorylated upon induction by hydrogen peroxide. Together, our results demonstrate that cells respond to oxidative stress via different signal transduction machinery dependent upon the nature of the oxidant.
机译：单倍体酿酒酵母中的交配发生在信息素反应途径激活后。该途径的生化成分参与其他酵母信号转导网络。为了更多地了解信号传导途径之间的协调，我们使用了一种“化学遗传”方法，寻找可以激活信息素应答基因FUS1和RLM1（细胞完整性途径的报告基因）的化合物。我们发现儿茶酚胺（L-3,4-羟苯丙氨酸[L-多巴]，多巴胺，肾上腺素和去甲肾上腺素）可提高FUS1和RLM1的转录。 N-乙酰半胱氨酸是酵母中的一种强大的抗氧化剂，完全逆转了这种作用，表明响应儿茶酚胺的FUS1和RLM1活化是氧化应激的结果。还发现氧化剂过氧化氢可激活RLM1报告基因的转录。进一步的遗传分析与免疫印迹相结合表明，交配的促分裂原活化蛋白激酶（MAPKs）之一Kss1和细胞完整性途径的MAPK Mpk1参与了L-多巴诱导的FUS1和RLM1转录的刺激。我们还报告说，Mpk1和Hog1，高渗透压MAPK，被过氧化氢诱导后被磷酸化。在一起，我们的结果表明细胞根据氧化剂的性质通过不同的信号转导机制对氧化应激作出反应。

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