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首页> 外文期刊>Molecular biology of the cell >Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology.
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Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology.

机译：正常液泡形态需要Luv1p / Rki1p / Tcs3p / Vps54p，一种定位于高尔基晚期和早期内体的酵母蛋白。

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摘要

We have characterized LUV1/RKI1/TCS3/VPS54, a novel yeast gene required to maintain normal vacuolar morphology. The luv1 mutant was identified in a genetic screen for mutants requiring the phosphatase calcineurin for vegetative growth. luv1 mutants lack a morphologically intact vacuole and instead accumulate small vesicles that are acidified and contain the vacuolar proteins alkaline phosphatase and carboxypeptidase Y and the vacuolar membrane H+-ATPase. Endocytosis appears qualitatively normal in luv1 mutants, but some portion (28%) of carboxypeptidase Y is secreted. luv1 mutants are sensitive to several ions (Zn2+, Mn2+, and Cd2+) and to pH extremes. These mutants are also sensitive to hygromycin B, caffeine, and FK506, a specific inhibitor of calcineurin. Some vacuolar protein-sorting mutants display similar drug and ion sensitivities, including sensitivity to FK506. Luv1p sediments at 100,000 x g and can be solubilized by salt or carbonate, indicating that it is a peripheral membrane protein. A Green Fluorescent Protein-Luv1 fusion protein colocalizes with the dye FM 4-64 at the endosome, and hemagglutinin-tagged Luv1p colocalizes with the trans-Golgi network/endosomal protease Kex2p. Computer analysis predicts a short coiled-coil domain in Luv1p. We propose that this protein maintains traffic through or the integrity of the early endosome and that this function is required for proper vacuolar morphology. [References: 86]
机译：我们已经表征了LUV1 / RKI1 / TCS3 / VPS54，一种维持正常液泡形态所需的新型酵母基因。 luv1突变体在遗传筛选中鉴定出需要磷酸酶钙调磷酸酶进行营养生长的突变体。 luv1突变体缺少形态完整的液泡，而是积聚了被酸化的小囊泡，其中包含液泡蛋白碱性磷酸酶和羧肽酶Y和液泡膜H + -ATPase。内吞作用在luv1突变体中定性正常，但羧肽酶Y的一部分（28％）被分泌。 luv1突变体对几种离子（Zn2 +，Mn2 +和Cd2 +）和极端pH敏感。这些突变体还对潮霉素B，咖啡因和钙调神经磷酸酶的特异性抑制剂FK506敏感。一些液泡蛋白分选突变体显示出相似的药物和离子敏感性，包括对FK506的敏感性。 Luv1p以100,000 x g沉淀，可以被盐或碳酸盐溶解，表明它是一种外周膜蛋白。绿色荧光蛋白-Luv1融合蛋白与染料FM 4-64在内体共定位，血凝素标记的Luv1p与反高尔基体/内体蛋白酶Kex2p共定位。计算机分析预测，Luv1p中的线圈线圈区域较短。我们建议这种蛋白质通过早期内体维持交通或完整性，并且此功能是适当的液泡形态所必需的。 [参考：86]

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