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首页> 外文期刊>Molecular biology of the cell >Recycling endosomes of polarized epithelial cells actively sort apical and basolateral cargos into separate subdomains
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Recycling endosomes of polarized epithelial cells actively sort apical and basolateral cargos into separate subdomains

机译：极化极化上皮细胞的回收内体主动将顶端和基底外侧货物分类为单独的亚域

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The plasma membranes of epithelial cells plasma membranes contain distinct apical and basolateral domains that are critical for their polarized functions. However, both domains are continuously internalized, with proteins and lipids from each intermixing in supranuclear recycling endosomes (REs). To maintain polarity, REs must faithfully recycle membrane proteins back to the correct plasma membrane domains. We examined sorting within REs and found that apical and basolateral proteins were laterally segregated into subdomains of individual REs. Subdomains were absent in unpolarized cells and developed along with polarization. Subdomains were formed by an active sorting process within REs, which precedes the formation of AP-1B-dependent basolateral transport vesicles. Both the formation of subdomains and the fidelity of basolateral trafficking were dependent on PI3 kinase activity. This suggests that subdomain and transport vesicle formation occur as separate sorting steps and that both processes may contribute to sorting fidelity.
机译：上皮细胞的质膜包含独特的顶端和基底外侧区域，这些区域对其极化功能至关重要。然而，两个结构域都被持续内在化，蛋白质和脂质各自混合在核回收内体（RE）中。为了保持极性，RE必须忠实地将膜蛋白循环回正确的质膜结构域。我们检查了RE中的排序，发现顶端和基底外侧蛋白被横向隔离到单个RE的亚域中。在非极化细胞中不存在亚域，并且随着极化而发展。子域是通过RE中的主动分选过程形成的，该过程先于AP-1B依赖性基底外侧转运囊泡的形成。亚结构域的形成和基底外侧运输的保真度均依赖于PI3激酶活性。这表明亚域和运输囊泡形成作为单独的分选步骤发生，并且这两个过程都可能有助于分选保真度。

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