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首页> 外文期刊>Molecular biology of the cell >Binding of CAP70 to inducible nitric oxide synthase and implications for the vectorial release of nitric oxide in polarized cells
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Binding of CAP70 to inducible nitric oxide synthase and implications for the vectorial release of nitric oxide in polarized cells

机译：CAP70与诱导型一氧化氮合酶的结合及其对极化细胞中一氧化氮矢量释放的影响

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摘要

In this article we analyze the mechanisms by which the C-terminal four amino acids of inducible nitric oxide synthase (NOS2) interact with proteins that contain PDZ (PSD-95/DLG/ZO-1) domains resulting in the translocation of NOS2 to the cellular apical domain. It has been reported that human hepatic NOS2 associates to EBP50, a protein with two PDZ domains present in epithelial cells. We describe herein that NOS2 binds through its four carboxy-terminal residues to CAP70, a protein that contains four PDZ modules that is targeted to apical membranes. Interestingly, this interaction augments both the cytochrome c reductase and (NO)-N-.-synthase activities of NOS2. Binding of CAP70 to NOS2 also results in an increase in the population of active NOS2 dimers. In addition, CAP70 participates in the correct subcellular targeting of NOS2 in a process that is also dependent on the acylation state of the N-terminal end of NOS2. Hence, nonpalmitoylated NOS2 is unable to progress toward the apical side of the cell despite its interaction with either EBP50 or CAP70. Likewise, if we abrogate the interaction of NOS2 with either EBP50 or CAP70 by fusing the GFP reporter to the carboxy-terminal end of NOS2 palmitoylation is not sufficient to confer an apical targeting.
机译：在本文中，我们分析了诱导型一氧化氮合酶（NOS2）C端四个氨基酸与包含PDZ（PSD-95 / DLG / ZO-1）域的蛋白质相互作用从而导致NOS2易位的机制。细胞顶端结构域。据报道，人肝NOS2与EBP50缔合，EBP50是一种在上皮细胞中具有两个PDZ结构域的蛋白质。我们在本文中描述了NOS2通过其四个羧基末端残基与CAP70结合，CAP70是一种蛋白质，其包含四个靶向顶膜的PDZ模块。有趣的是，这种相互作用既增强了细胞色素C还原酶的活性，又增强了NOS2的（NO）-N-。合酶的活性。 CAP70与NOS2的结合也导致活性NOS2二聚体的数量增加。另外，CAP70以还依赖于NOS2的N-末端的酰化状态的过程参与NOS2的正确的亚细胞靶向。因此，尽管非棕榈酰化的NOS2与EBP50或CAP70相互作用，却无法向细胞的顶端发展。同样，如果我们通过将GFP报告基因融合到NOS2的棕榈酸酯化的羧基末端来消除NOS2与EBP50或CAP70的相互作用，则不足以赋予其根尖靶向性。

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