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首页> 外文期刊>Molecular biology of the cell >Association of ARVCF with zonula occludens (ZO)-1 and ZO-2: Binding to PDZ-domain proteins and cell-cell adhesion regulate plasma membrane and nuclear localization of ARVCF
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Association of ARVCF with zonula occludens (ZO)-1 and ZO-2: Binding to PDZ-domain proteins and cell-cell adhesion regulate plasma membrane and nuclear localization of ARVCF

机译：ARVCF与小带闭塞（ZO）-1和ZO-2的关联：与PDZ域蛋白的结合和细胞间粘附力调节质膜和ARVCF的核定位

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摘要

ARVCF, an armadillo-repeat protein of the p120(ctn) family, associates with classical cadherins and is present in adherens junctions, but its function is poorly understood. Here, we show that ARVCF interacts via a C-terminal PDZ-binding motif with zonula occludens (ZO)-1 and ZO-2. ARVCF and ZO-1 partially colocalize in the vicinity of the apical adhesion complex in polarized epithelial Madin-Darby canine kidney cells. ARVCF, ZO-1, and E-cadherin form a complex and are recruited to sites of initial cell-cell contact in sparse cell cultures. E-cadherin binding and plasma membrane localization of ARVCF require the PDZ-binding motif. Disruption of cell-cell adhesion releases ARVCF from the plasma membrane and an increased fraction of the protein localizes to the nucleus. Nuclear localization of ARVCF also requires the PDZ-binding motif and can be mediated by the PDZ domains of ZO-2. Thus, the interaction of ARVCF with distinct PDZ-domain proteins determines its subcellular localization. Interactions with ZO-1 and ZO-2, in particular, may mediate recruitment of ARVCF to the plasma membrane and the nucleus, respectively, possibly in response to cell-cell adhesion cues.
机译：ARVCF是p120（ctn）家族的犰狳重复蛋白，与经典的钙粘蛋白有关，并存在于粘附连接中，但对其功能的了解甚少。在这里，我们显示ARVCF通过C端PDZ结合基序与小带闭塞（ZO）-1和ZO-2相互作用。 ARVCF和ZO-1在极化的上皮Madin-Darby犬肾细胞中的根尖黏附复合体附近部分共定位。 ARVCF，ZO-1和E-钙粘蛋白形成复合物，并被募集到稀疏细胞培养物中初始细胞与细胞接触的位置。 E-钙粘着蛋白结合和ARVCF的质膜定位需要PDZ结​​合基序。细胞间粘附的破坏会从质膜释放ARVCF，并且增加的蛋白质部分会定位于细胞核。 ARVCF的核定位也需要PDZ结​​合基序，并且可以由ZO-2的PDZ域介导。因此，ARVCF与不同的PDZ域蛋白的相互作用决定了其亚细胞定位。特别是，与ZO-1和ZO-2的相互作用可能会介导ARVCF募集到质膜和细胞核，可能是由于细胞与细胞之间的粘附反应。

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