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首页> 外文期刊>Molecular biology of the cell >Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10+
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Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10+

机译：裂变酵母中的纺锤极体复制发生在G1 / S边界，但成熟被阻止，直到由于cdc10 +下游事件从S退出

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摘要

The regulation and timing of spindle pole body (SPB) duplication and maturation in fission yeast was examined by transmission electron microscopy. When cells are arrested at G1 by nitrogen starvation, the SPB is unduplicated. On release from G1, the SPBs were duplicated after 1-2 h. In cells arrested at S by hydroxyurea, SPBs are duplicated but not mature. In G1 arrest/release experiments with cdc2.33 cells at the restrictive temperature, SPBs remained single, whereas in cells at the permissive temperature, SPBs were duplicated. In cdc10 mutant cells, the SPBs seem not only to be duplicated but also to undergo partial maturation, including invagination of the nuclear envelope underneath the SPB. There may be an S-phase-specific inhibitor of SPB maturation whose expression is under control of cdc10(+). This model was examined by induction of overreplication of the genome by overexpression of rum1p or cdc18p. In cdc18p-overexpressing cells, the SPBs are duplicated but not mature, suggesting that cdc18p is one component of this feedback mechanism. In contrast, cells overexpressing rum1p have large, deformed SPBs accompanied by other features of maturation and duplication. We propose a feedback mechanism for maturation of the SPB that is coupled with exit from S to trigger morphological changes.
机译：通过透射电子显微镜检查了裂变酵母中纺锤极体（SPB）复制和成熟的调控和时间。当细胞因氮饥饿而停滞在G1时，SPB不会复制。从G1释放后，SPB会在1-2小时后复制一次。在被羟基脲阻滞于S的细胞中，SPBs复制但不成熟。在限制温度下使用cdc2.33细胞进行的G1阻滞/释放实验中，SPB保持单一状态，而在允许温度下的细胞中，SPB重复存在。在cdc10突变细胞中，SPB似乎不仅要复制，而且还要经历部分成熟，包括SPB下方核膜的内陷。可能是SPB成熟的S期特异性抑制剂，其表达受cdc10（+）控制。通过rum1p或cdc18p的过表达诱导基因组的过度复制来检查该模型。在过量表达cdc18p的细胞中，SPB是重复的但不成熟，这表明cdc18p是这种反馈机制的一个组成部分。相反，过度表达rum1p的细胞具有较大的变形SPB，并伴有其他成熟和复制特征。我们提出了一种反馈机制，用于使SPB成熟，并与S的出口耦合以触发形态变化。

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