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首页> 外文期刊>Molecular biology reports >Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis
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Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis

机译：基于ISSR分析的皖北桔梗种质资源评价

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Based on genetic diversity analysis with ISSR-PCR, this study was to access the germplasm resources of Platycodon grandiflorum in northern Anhui province. Ten primers that could produce more distinct and repeatable bands were used for ISSR-PCR. Statistic analysis was conducted by POPGENE v. 1.32, Arlequin3.1, NTSYS-pc version 2.1. (1) Seventy-four polymorphic bands (76.29 %) out of a total of 97 were generated from 105 individuals in five populations. (2) Shannon index of diversity ranged from 0.307 to 0.260, diversity at species level was 0.3581, which means superior genetic diversity. (3) Genetic diversity across all the populations revealed by AMOVA indicated that 86.02 % occurred within populations. (4) The Fst value was 0.1398, indicating a intermediate genetic differentiation among populations. (5) Dendrogram relationship illustrated genetic distance was correlated with geographic distance. ISSR markers can be used for studying genetic diversity of P. grandiflorum. Degradation of populations doesn't happen in northern Anhui province, bank of germplasm preservation should be established for cultivation of excellent variety of P. grandiflorum.
机译：基于ISSR-PCR的遗传多样性分析，本研究旨在获取皖北桔梗种质资源。 ISSR-PCR使用了十个可能产生更多不同和可重复条带的引物。统计分析由POPGENE v.1.32，Arlequin3.1，NTSYS-pc版本2.1进行。 （1）从五个群体的105个个体中产生了97个基因中的74个多态性谱带（76.29％）。 （2）Shannon多样性指数在0.307至0.260之间，物种水平的多样性为0.3581，这意味着优良的遗传多样性。 （3）AMOVA揭示的所有种群的遗传多样性表明，种群内部有86.02％的发生。 （4）Fst值为0.1398，表明种群间存在中等遗传分化。 （5）树状图关系说明遗传距离与地理距离相关。 ISSR标记可用于研究大花。草的遗传多样性。皖北地区不会发生种群退化的情况，应建立种质保藏库，以培育优良的P. grandiflorum。

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