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首页> 外文期刊>Molecular biology of the cell >Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
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Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1

机译：内体SNARE蛋白syntaxin-8和钾通道TASK-1的协同胞吞作用

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The endosomal SNARE protein syntaxin-8 interacts with the acid-sensitive potassium channel TASK-1. The functional relevance of this interaction was studied by heterologous expression of these proteins (and mutants thereof) in Xenopus oocytes and in mammalian cell lines. Coexpression of syntaxin-8 caused a fourfold reduction in TASK-1 current, a corresponding reduction in the expression of TASK-1 at the cell surface, and a marked increase in the rate of endocytosis of the channel. TASK-1 and syntaxin-8 colocalized in the early endosomal compartment, as indicated by the endosomal markers 2xFYVE and rab5. The stimulatory effect of the SNARE protein on the endocytosis of the channel was abolished when both an endocytosis signal in TASK-1 and an endocytosis signal in syntaxin-8 were mutated. A syntaxin-8 mutant that cannot assemble with other SNARE proteins had virtually the same effect as wild-type syntaxin-8. Total internal reflection fluorescence microscopy showed formation and endocytosis of vesicles containing fluorescence-tagged clathrin, TASK-1, and/or syntaxin-8. Our results suggest that the unassembled form of syntaxin-8 and the potassium channel TASK-1 are internalized via clathrin-mediated endocytosis in a cooperative manner. This implies that syntaxin-8 regulates the endocytosis of TASK-1. Our study supports the idea that endosomal SNARE proteins can have functions unrelated to membrane fusion.
机译：内体SNARE蛋白syntaxin-8与酸敏感性钾通道TASK-1相互作用。通过在爪蟾卵母细胞和哺乳动物细胞系中这些蛋白质（及其突变体）的异源表达研究了这种相互作用的功能相关性。 Syntaxin-8的共表达导致TASK-1电流减少四倍，相应地，TASK-1在细胞表面的表达减少，并且通道内吞率显着增加。如内体标记2xFYVE和rab5所示，TASK-1和Syntaxin-8共定位在早期的内体区室中。当TASK-1中的内吞信号和Syntaxin-8中的内吞信号均发生突变时，SNARE蛋白对通道内吞的刺激作用就消失了。不能与其他SNARE蛋白质组装的syntaxin-8突变体实际上具有与野生型syntaxin-8相同的效果。全内反射荧光显微镜显示含有荧光标记网格蛋白，TASK-1和/或Syntaxin-8的囊泡的形成和内吞作用。我们的研究结果表明，语法蛋白8和钾通道TASK-1的未组装形式通过网格蛋白介导的内吞作用以协同方式被内化。这暗示语法语法8调节TASK-1的胞吞作用。我们的研究支持内体SNARE蛋白可以具有与膜融合无关的功能这一想法。

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