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首页> 外文期刊>Molecular biology reports >Isocitrate lyase is required for urediniospore germination of Puccinia striiformis f. sp tritici
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Isocitrate lyase is required for urediniospore germination of Puccinia striiformis f. sp tritici

机译：小麦条锈菌的孢子萌发需要异柠檬酸裂合酶。小麦

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摘要

The PstICL1 gene, which encodes isocitrate lyase, a key enzyme in the glyoxylate cycle, was cloned and characterized in the biotrophic wheat pathogen Puccinia striiformis f. sp. tritici (Pst). Expression analyses of PstICL1 exhibited high levels of transcripts in ungerminated urediniospores, which showed low isocitrate lyase enzyme activity. In planta, PstICL1 expression was continuously down-regulated upon germination. During the later stages of the infection of wheat, the level of PstICL1 expression was extremely low. The function of PstICL1 was identified via mutant complementation. The expression of PstICL1 in Saccharomyces cerevisiae can complement the defects of the Delta ICL mutant. Using 3-nitropropionate, we observed that inactivation of isocitrate lyase greatly reduced the germination rate of urediniospores, indicating that PstICL1 plays a key role during Pst germination. Furthermore, analysis of lipid bodies revealed that lipid components continuously enter the germ tube from the urediniospore cell during germ tube elongation. Moreover, during this period, the lipid contents continuously decreased, and the total carbohydrates markedly increased, demonstrating that the lipids are being converted into carbohydrates. These results suggest that PstICL1 is required for Pst germination.
机译：编码异柠檬酸裂合酶（乙醛酸循环中的关键酶）的PstICL1基因被克隆并鉴定为生物营养型小麦病原体Puccinia striiformis f。 sp。 Tritici（Pst）。 PstICL1的表达分析在未发芽的梭孢子孢子中显示高水平的转录本，这表明低的异柠檬酸裂合酶活性。在植物中，发芽后PstICL1的表达持续下调。在小麦感染的后期，PstICL1表达水平极低。通过突变互补来鉴定PstICL1的功能。 PstICL1在酿酒酵母中的表达可以弥补Delta ICL突变体的缺陷。使用3-硝基丙酸酯，我们观察到异柠檬酸裂合酶的失活大大降低了脲原孢子的发芽率，表明PstICL1在Pst萌发过程中起关键作用。此外，对脂质体的分析表明，在胚芽管伸长过程中，脂质成分不断地从脲原孢子细胞进入胚芽管。此外，在此期间，脂质含量持续降低，并且总碳水化合物显着增加，表明脂质正在转化为碳水化合物。这些结果表明PstICL1是Pst发芽所必需的。

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