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首页> 外文期刊>Molecular biology reports >Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis.
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Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis.

机译:通过使用大量分离剂分析鉴定与玉米抗南方玉米叶枯病的rhm基因紧密相关的AFLP标记。

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摘要

Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB. One co-dominant AFLP marker, p7m36, was mapped to a position 1.0 cM from rhm, and we converted this marker to an STS (sequence-tagged site) marker. Combined with the previously identified agrP144, this new marker may be useful for map-based cloning of the rhm gene and marker-assisted selection for rhm.
机译:南部玉米叶枯病(SCLB)是全世界温带和热带玉米产区的重要疾病。我们将扩增的片段长度多态性(AFLP)技术和大体积分离子分析(BSA)结合应用到一个大的F2种群中,以鉴定与rhm基因相关的分子标记,以抵抗SCLB。一个共同的AFLP标记p7m36被定位到距rhm 1.0 cM的位置,然后我们将该标记转换为STS(序列标记位点)标记。与先前鉴定的agrP144结合使用,这种新标记可能可用于基于图的rhm基因克隆和标记辅助的rhm选择。

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