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首页> 外文期刊>Molecular biology reports >Genomic analysis of cultivated barley (Hordeum vulgare) using sequence-tagged molecular markers. Estimates of divergence based on RFLP and PCR markers derived from stress-responsive genes, and simple-sequence repeats (SSRs)
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Genomic analysis of cultivated barley (Hordeum vulgare) using sequence-tagged molecular markers. Estimates of divergence based on RFLP and PCR markers derived from stress-responsive genes, and simple-sequence repeats (SSRs)

机译：使用序列标签的分子标记对栽培大麦（大麦）进行基因组分析。基于源自压力响应基因的RFLP和PCR标记以及简单序列重复（SSR）的差异估计

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Three types of molecular markers have been compared for their utility in evaluating genetic diversity among cultivars of Hordeum vulgare. Restriction fragment length polymorphisms at 71 sites were scored with the aid of probes corresponding to stress-responsive genes from barley and wheat, coding for a low-molecular-weight heat shock protein, a dehydrin, an aldose reductase homolog, and a 18.9-kDa drought-induced protein of unknown function. Indexes of genetic diversity computed in the total sample and within groups of cultivars (two-rowed and six-rowed, winter and spring varieties) indicated high values of genetic differentiation (F_(ST) > 15%). A second assessment of genetic diversity was performed by PCR amplification of genomic DNA using as primers 13 arbitrary oligonucleotides derived from sequences of the same stress-responsive genes. A high degree of polymorphism was uncovered using these markers also, but they yielded low values for F_(ST) (< 7%) among groups of cultivars. Finally, 15 different simplesequence repeats (AC or AG) were amplified with primers based on unique flanking sequences. Levels of polymorphism and differentiation between groups of cultivars revealed by these markers were quite high. Ordination techniques applied to measures of genetic distance among cultivars demonstrated a remarkable ability of the RFLPs associated with stress-responsive genes to discriminate on the basis of growth habit. The correlation with production data for the cultivars in different environments was also significant. This "functional genomics" strategy was therefore as informative as the "structural genomics" (SSR-based) approach, but requires the analysis of fewer probes.
机译：比较了三种类型的分子标记在评估大麦品种遗传多样性方面的效用。借助于对应于来自大麦和小麦的应激反应基因的探针对71个位点的限制性片段长度多态性进行评分，该基因编码低分子量热休克蛋白，脱水素，醛糖还原酶同源物和18.9-kDa干旱诱导的功能未知的蛋白质。在总样本和品种组（两行和六行，冬季和春季品种）中计算的遗传多样性指数表明遗传分化值很高（F_（ST）> 15％）。遗传多样性的第二次评估是通过使用13种衍生自相同胁迫响应基因序列的任意寡核苷酸作为引物，对基因组DNA进行PCR扩增来进行的。使用这些标记物也未发现高度的多态性，但在不同品种的组中，它们的F_（ST）值较低（<7％）。最后，使用基于独特侧翼序列的引物扩增了15个不同的简单序列重复序列（AC或AG）。这些标记揭示的品种组之间的多态性和分化水平很高。用于种间遗传距离测量的排序技术表明，与胁迫响应基因相关的RFLP具有显着的能力，可根据生长习性进行区分。与不同环境中的品种的生产数据的相关性也很重要。因此，这种“功能基因组学”策略与“结构基因组学”（基于SSR）方法一样有用，但是需要分析更少的探针。

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