Isolation of High Quality RNA from Bilberry (Vaccinium myrtillus L.) Fruit

Laura Jaakola; Anna Maria Pirttila; Minna Halonen; Anja Hohtola

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Isolation of High Quality RNA from Bilberry (Vaccinium myrtillus L.) Fruit

机译：越桔果实中高质量RNA的分离

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A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and #beta#-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.

机译：描述了一种简单有效的方法，用于从越橘果实中分离高质量的RNA。该程序基于在提取缓冲液中使用十六烷基三甲基溴化铵（CTAB），聚乙烯吡咯烷酮（PVP）和＃β＃-巯基乙醇，以消除多糖并防止酚类化合物的氧化。该方法是对松树所述方法的一种改进，可产生适用于基于cDNA方法的高质量RNA。该方法适用于多种植物组织。

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来源
《Molecular biotechnology》 |2001年第2期|共3页
作者
Laura Jaakola; Anna Maria Pirttila; Minna Halonen; Anja Hohtola;
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原文格式 PDF
正文语种 eng
中图分类分子生物学;生物工程学（生物技术）;
关键词
RNA isolation; CTAB; fruit tissue; bilberry; vaccinium myrtillus;

机译：RNA分离;CTAB;水果组织;越桔;牛痘苗;

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专利

1. Isolation of High Quality RNA from Bilberry (Vaccinium myrtillus L.) Fruit [J] . Laura Jaakola, Anna Maria Pirttila, Minna Halonen, Molecular biotechnology . 2001,第2期

机译：越桔果实中高质量RNA的分离
2. White versus blue: Does the wild 'albino' bilberry (Vaccinium myrtillus L.) differ in fruit quality compared to the blue one? [J] . Zorenc Zala, Veberic Robert, Stampar Franci, Food Chemistry . 2016,第nova15期

机译：白色与蓝色：与蓝色相比，野生“白化”越桔（Vaccinium myrtillus L.）的果实品质是否有所不同？
3. A comparison of fruit quality parameters of wild bilberry (Vaccinium myrtillus L.) growing at different locations [J] . Mikulic-Petkovsek Maja, Schmitzer Valentina, Slatnar Ana, Journal of the Science of Food and Agriculture . 2015,第4期

机译：不同位置生长的野生越桔（Vaccinium myrtillus L.）果实品质参数的比较
4. An improved method of isolation of high quality total RNA from purple-fleshed sweetpotato,Ipomoea batatas(L.)Lam. [C] . Wei Zhou, Yifu Gong, Qili Feng, 3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)论文集 . 2008

机译：一种从紫色肉甘薯（Ipomoea batatas（L.）Lam）分离高质量总RNA的改进方法。
5. Gaseous storage effects on fruit quality, shelf life, and some pathogens on 'Tifblue' blueberry (Vaccinium ashei L.). [D] . Tokitkla, Angsana. 2004

机译：气体存储对果实品质，保质期和“ Tifblue”蓝莓（越橘越桔）的某些病原体有影响。
6. Black Currant (Ribes nigrum L.) and Bilberry (Vaccinium myrtillus L.) Fruit Juices Inhibit Adhesion of Asaia spp. [O] . Hubert Antolak, Agata Czyzowska, Dorota Kregiel 2006

机译：黑加仑（Ribes nigrum L.）和越桔（Vaccinium myrtillus L.）果汁抑制Asaia spp的粘附。
7. Zmienność produkcji owoców borówki czernicy (Vaccinium myrtillus L.) na tle zróżnicowania fitosocjologicznego borów w Puszczy Białowieskiej [Variability of fruit production by bilberries (Vaccinium myrtillus L.) in relation to the phytosociological differentiation in the coniferous forests of the Białowieża Forest] [O] . K. Falińska 2015

机译：Zmiennośćprodukcjomeomówobówkiczernicy（Vaccinium myrtillus L.）natlezróżnicowaniafitosocjologicznegoborówwpuszczyBiałowieskiej[越桔（Vaccinium myrtillus L.）水果生产的变异与Białowieża森林针叶林的植物生态分化有关]

Isolation of High Quality RNA from Bilberry (Vaccinium myrtillus L.) Fruit

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