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首页> 外文期刊>Molecular biotechnology >Half-embryo cocultivation technique for estimating the susceptibility of pea (Pisum sativum L.) and lentil (Lens culinaris Medik.) cultivars to Agrobacterium tumefaciens.
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Half-embryo cocultivation technique for estimating the susceptibility of pea (Pisum sativum L.) and lentil (Lens culinaris Medik.) cultivars to Agrobacterium tumefaciens.

机译:半胚共培养技术,用于估计豌豆(Pisum sativum L.)和小扁豆(Lens culinaris Medik。)品种对根癌农杆菌的敏感性。

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摘要

Longitudinally sliced embryonic axes from pea and lentil mature seeds cocultivated with A. tumefaciens carrying a gus reporter gene in its T-DNA provided a convenient means to evaluate the efficiency of gene transfer to tissues in different cultivars and cocultivation conditions. Use of this technique demonstrated wide variation in susceptibility to Agrobacterium among several pea and lentil commercial genotypes.
机译:从豌豆和小扁豆成熟种子的经纵向切片的胚轴与在其T-DNA中携带gus报道基因的根癌农杆菌共培养,为评估基因在不同品种和共培养条件下转移至组织的效率提供了便利的手段。这项技术的使用证明了几种豌豆和小扁豆商业基因型对农杆菌的敏感性差异很大。

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