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首页> 外文期刊>Molecular medicine reports >Sasanquasaponin from Camellia oleifera Abel. induces apoptosis via Bcl-2, Bax and caspase-3 activation in HepG2 cells
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Sasanquasaponin from Camellia oleifera Abel. induces apoptosis via Bcl-2, Bax and caspase-3 activation in HepG2 cells

机译：茶油中的Sasanquasaponin。通过HepG2细胞中的Bcl-2，Bax和caspase-3激活诱导凋亡

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摘要

The present study aimed to elucidate the molecular mechanisms underlying the induction of cytotoxic effects by sasanquasaponin (SQS) in HepG2 cells. Following SQS treatment, time- and dose-dependent increases in the apoptotic rate were observed. The induction of cell death by SQS mainly occurs via programmed cell death, as indicated by Annexin V-fluorescein isothiocyanate and propidium iodide staining, where up to 30% apoptotic cells were detected following 12 h SQS treatment. Reverse transcription-poly-merase chain reaction analysis demonstrated that SQS treatment upregulated B-cell lymphoma-2 (Bcl-2)-associated x protein and caspase-3 mRNA expression and downregulated Bcl-2 mRNA expression. Greater alterations in Bax, Bcl-2 and caspase-3 expression were observed with increasing treatment duration. The decrease in Bcl-2, increase in Bax and, finally, the activation of caspase-3 in HepG2 cells indicated that the apoptotic process induced by SQS was irreversible. The results of the present study therefore suggested that SQS induced HepG2 cell apoptosis via the activation of mitochondrial apoptotic pathways.
机译：本研究旨在阐明Sasaquasaponin（SQS）在HepG2细胞中诱导细胞毒性作用的分子机制。 SQS处理后，观察到凋亡率随时间和剂量的增加。 SQS诱导的细胞死亡主要通过程序性细胞死亡发生，如膜联蛋白V-异硫氰酸荧光素和碘化丙啶染色所示，SQS处理12 h后可检测到高达30％的凋亡细胞。逆转录聚合酶链反应分析表明，SQS治疗上调了B细胞淋巴瘤2（Bcl-2）相关的x蛋白和caspase-3 mRNA表达，下调了Bcl-2 mRNA表达。随着治疗时间的延长，观察到Bax，Bcl-2和caspase-3表达的更大改变。 Bcl-2的减少，Bax的增加以及最后HepG2细胞中caspase-3的激活表明SQS诱导的凋亡过程是不可逆的。因此，本研究结果表明，SQS通过激活线粒体凋亡途径诱导HepG2细胞凋亡。

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