...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular medicine reports >Repair of mandibular defects by bone marrow stromal cells expressing the basic fibroblast growth factor transgene combined with multi-pore mineralized Bio-Oss
【24h】

Repair of mandibular defects by bone marrow stromal cells expressing the basic fibroblast growth factor transgene combined with multi-pore mineralized Bio-Oss

机译：表达碱性成纤维细胞生长因子转基因的骨髓基质细胞结合多孔矿化Bio-Oss修复下颌骨缺损

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of the present study was to evaluate the effect of combining Bio-Oss with bone marrow stromal cells (BMSCs) transfected with the basic fibroblast growth factor (bFGF) gene on bone regeneration during mandibular distraction of rabbits. BMSCs obtained from rabbits were transfected with bFGF gene-encoding plasmids and proliferation rate and the differentiation marker alkaline phosphatase activity were measured. Following seeding into Bio-Oss collagen and 9-day culture in vitro, the surface morphology of the Bio-Oss was assessed using scanning electron microscopy analysis. Three mandibular defects were induced in the lower border of the bilateral mandibular ramus in each New Zealand white rabbit (total n=6). Three scaffolds, group A (seeded with BMSCs/bFGF), B (seeded with BMSCs/pVAX1) and C (cell-free), which had been cultured in vitro under standard cell culture conditions for 18 days, were implanted into mandibular defects under sterile conditions. Animals were sacrificed by anesthesia overdose 12 weeks following surgery and the scaffolds were extracted for bone mineral density and histological analyses. Results indicate that bFGF was successfully transfected into BMSCs. Proliferation and osteoblast differentiation of BMSCs were stimulated by bFGE: in vitro. No differences were identified in surface morphology for Bio-Oss loaded with variable groups of cells. At week 12 following implantation of Bio-Oss scaffolds, mineralization of BMSCs in Bio-Oss scaffolds was observed to be increased by bFGF. New bone and cartilage formation was revealed in hematoxylin and eosin-stained sections in Bio-Oss scaffolds and was most abundant in group A (BMSCs transfected with bFGF). In the current study, the bFGF gene was transfected into BMSCs and expressed successfully. bFGF promoted proliferation and differentiation of BMSCs in vitro and implantation of bFGF-expressing BMSCs combined with Bio-Oss enhanced new bone regeneration more effectively than traditional methods.
机译：本研究的目的是评估Bio-Oss与转染碱性成纤维细胞生长因子（bFGF）基因的骨髓基质细胞（BMSCs）联合对兔下颌骨牵张过程中骨再生的影响。用bFGF基因编码质粒转染从兔获得的BMSC，并测定其增殖速率和分化标记碱性磷酸酶活性。在植入Bio-Oss胶原蛋白并进行9天体外培养后，使用扫描电子显微镜分析评估了Bio-Oss的表面形态。在每只新西兰大白兔的双侧下颌支的下缘诱发了三个下颌缺损（总计n = 6）。将在标准细胞培养条件下体外培养18天的三个支架A组（BMSCs / bFGF接种），B（BMSCs / pVAX1接种）和C（无细胞）支架植入下颌缺损下无菌条件。在手术后12周通过麻醉过量处死动物，并提取支架以进行骨矿物质密度和组织学分析。结果表明，bFGF已成功转染到BMSCs中。 bFGE：在体外刺激BMSCs的增殖和成骨细胞分化。对于装载有可变细胞组的Bio-Oss，在表面形态上没有发现差异。在植入Bio-Oss支架后的第12周，观察到bFGF可增加Bio-Oss支架中BMSC的矿化。在Bio-Oss支架的苏木精和曙红染色切片中发现了新的骨骼和软骨形成，并且在A组中最为丰富（BMSC转染了bFGF）。在当前的研究中，bFGF基因被转染到BMSCs中并成功表达。 bFGF促进了体外BMSCs的增殖和分化，表达bFGF的BMSCs与Bio-Oss的结合比传统方法更有效地促进了新骨再生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号