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首页> 外文期刊>Moscow University Soil Science Bulletin >Agrobacterium tumefaciens as Associative Nitrogen-Fixing Bacteria
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Agrobacterium tumefaciens as Associative Nitrogen-Fixing Bacteria

机译：根癌农杆菌作为固氮细菌

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摘要

From the root surface of vegetable plants growing in the soils of North Vietnam, 227 pure bacterial cultures are isolated on nitrogen-free media. All the cultures show nitrogen-fixing ability and positive effect on plant growth in model experiments, while none of them cause the formation of root nodules or tumors. Based on the gene sequencing of the 16S rRNA, our determination of the phylogenic position of the most active isolated strains reveals their affiliation with the family Rhizobiaceae of thephylum Alpharoteobacteria and that they are closest to the type strain of the genus Agrobacterium—A. tumefaciens. The physiology and morphological features also indicate the affiliation of these organisms with A. tumefaciens. Thus, our isolated strain of this plant pathogen bacterium appears as associative diazotrophs, which also have a positive effect on the growth of cultural plants.
机译：从在越南北部土壤中生长的蔬菜植物的根表面，在无氮培养基上分离出227种纯细菌培养物。在模型实验中，所有培养物均显示出固氮能力并对植物的生长产生积极影响，而它们均未引起根瘤或肿瘤的形成。根据16S rRNA的基因测序，我们确定了最活跃的分离菌株的系统发生位置，揭示了它们与Alpharoteobacteriaphylphyleobacteria根瘤菌科的隶属关系，并且它们与农杆菌属A型菌株最接近。根瘤菌。生理学和形态学特征也表明这些生物与根癌农杆菌的联系。因此，我们分离出的该植物病原菌菌株显示为缔合重氮菌，也对培养植物的生长具有积极作用。

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