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首页> 外文期刊>Molecular ecology >Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera : Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction
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Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera : Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction

机译：基于随机扩增多态性DNA-聚合酶链反应的烟粉虱伊比利亚种群的遗传多样性

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摘要

The genetic structure of six Iberian populations of the whitefly Bemisia tabaci, two of them biotype Q, one biotype B, and the other three a mixture of both, has been studied using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total of 336 individuals was completely discriminated by means of 234 scored bands. Separate analyses of molecular variance of haploid males and diploid females using the pairwise number of differences between haplotypes showed that biotypes contribute significantly more to the observed variability than populations within biotypes. On average, gene flow between two biotypes of the same population is lower than between populations of identical biotypes. On the basis of these results and the nondetection under natural conditions of a single hybrid, we consider that both biotypes are genetically isolated under the ecological conditions prevailing in the south Iberian Peninsula. All populations of biotype Q presented similar values of intrapopulational diversity, which were higher than the values shown by populations of biotype B. [References: 38]
机译：使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）研究了六个粉虱烟粉虱的伊比利亚种群的遗传结构，其中两个是Q型，一个是B型，另外三个是两者的混合物。通过234个计分频段，完全区分了336个人。使用单倍型之间的成对差异对单倍体雄性和二倍体雌性的分子方差进行单独分析，结果表明，生物型对观察到的变异性的贡献远大于生物型内的种群。平均而言，相同种群的两种生物类型之间的基因流低于相同生物类型的种群之间的基因流。根据这些结果以及在自然条件下没有发现单个杂种的情况，我们认为两种生物型都是在伊比利亚南部半岛普遍存在的生态条件下遗传分离的。所有Q型生物种群均表现出相似的种群内多样性值，高于B型生物种群显示的值。[参考文献：38]

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