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首页> 外文期刊>Molecular ecology notes >Isolation and characterization of simple sequence repeat loci in Rubus hochstetterorum and their use in other species from the Rosaceae family
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Isolation and characterization of simple sequence repeat loci in Rubus hochstetterorum and their use in other species from the Rosaceae family

机译：悬钩子中简单序列重复基因座的分离，鉴定及其在蔷薇科其他物种中的应用

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摘要

The identification of microsatellite loci in Rubus hochstetterorum provides an important tool for the characterization and conservation of wild populations of this species. Cross-species amplification of markers may be of particular interest for the study of other Rubus species. In this study, 41 simple sequence repeat markers were identified in a genomic library of R. hochstetterorum. Fifteen of the identified microsatellite loci were characterized in a set of 30 samples and revealed to be polymorphic with three to 19 alleles per locus. All the identified markers allowed cross-species amplification in at least one of the other three tested species from the Rosaceae family.
机译：悬钩子中微卫星基因座的鉴定为表征和保护该物种的野生种群提供了重要的工具。标记物的跨物种扩增可能对研究其他悬钩子属物种特别感兴趣。在这项研究中，在R. hochstetterorum基因组文库中鉴定出41个简单的序列重复标记。在一组30个样本中对15个已鉴定的微卫星基因座进行了特征分析，发现每个基因座具有3至19个等位基因。所有鉴定出的标记均允许在蔷薇科其他三个被测物种中的至少一个物种中进行跨物种扩增。

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