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首页> 外文期刊>Molecular ecology notes >Isolation and characterization of microsatellite markers from Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch), and cross-amplification in the closely related species crucian carp, Carassius auratus auratus (Linnaeus)
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Isolation and characterization of microsatellite markers from Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch), and cross-amplification in the closely related species crucian carp, Carassius auratus auratus (Linnaeus)

机译:方正银cru鱼Car鱼微卫星标记的分离和鉴定,以及与related鱼密切相关的Car鱼cross鱼的交叉扩增

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摘要

We report the development of 20 microsatellite markers for Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch). Nineteen out of 20 showed polymorphism with alleles ranging from two to 14. These loci were screened to amplify the closely related species crucian carp, Carassius auratus auratus (Linnaeus) and all of them can amplify DNA with the size similar to the former. The origin of silver crucian carp is in issue and the population genetic structure is still unclear. Microsatellite markers isolated from the silver crucian carp and their utility in the crucian carp will be useful for these researches.
机译:我们报告了方正银cru鱼(Carassius auratus gibelio(Bloch))的20个微卫星标记的发展。 20个基因中有19个具有等位基因,介于2至14个等位基因之间。这些位点经过筛选,以扩增密切相关的Car鱼Car鱼(Carassius auratus auratus,Linnaeus),它们都可以扩增大小与前者相似的DNA。 cru鱼的起源已成问题,种群遗传结构仍不清楚。从the鱼中分离的微卫星标记及其在the鱼中的应用将对这些研究有用。

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