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首页> 外文期刊>Molecular oral microbiology >Porphyromonas gingivalis gingipain is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm
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Porphyromonas gingivalis gingipain is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm

机译：牙龈卟啉单胞菌牙龈卟啉单胞菌参与了放线菌放线菌生物膜的分离和聚集

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Summary: Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are major periodontal pathogens that cause several types of periodontal disease. Our previous study suggested that P. gingivalis gingipains secreted in the subgingival environment are related to the detachment of A.actinomycetemcomitans biofilms. However, it remains unclear whether arginine-specific cysteine proteinase (Rgp) and lysine-specific proteinase (Kgp) play different roles in the detachment of A. actinomycetemcomitans biofilm. The aim of this study was to investigate possible disruptive roles of Kgp and Rgp in the aggregation and attachment of A. actinomycetemcomitans. While P. gingivalis ATCC33277 culture supernatant has an ability to decrease autoaggregation and coaggregation of A. actinomycetemcomitans cells, neither the boiled culture supernatant of ATCC33277 nor the culture supernatant of KDP136 showed this ability. The addition of KYT-1 and KYT-36, specific inhibitors of Rgp and Kgp, respectively, showed no influence on the ability of P. gingivalis culture supernatant. The result of gelatin zymography suggested that other proteases processed by gingipains mediated the decrease of A. actinomycetemcomitans aggregations. We also examined the biofilm-destructive effect of gingipains by assessing the detachment of A. actinomycetemcomitans from polystyrene surfaces. Scanning electron microscope analysis indicated that A. actinomycetemcomitans cells were detached by P. gingivalis Kgp. The quantity of A. actinomycetemcomitans in biofilm was decreased in co-culture with P. gingivalis. However, this was not found after the addition of KYT-36. These findings suggest that Kgp is a critical component for the detachment and decrease of A. actinomycetemcomitans biofilms.
机译：摘要：牙龈卟啉单胞菌和放线杆菌聚集体是引起几种类型牙周疾病的主要牙周病原体。我们先前的研究表明，龈下环境中分泌的牙龈卟啉单胞菌姜黄素与放线放线杆菌生物膜的分离有关。但是，尚不清楚精氨酸特异性半胱氨酸蛋白酶（Rgp）和赖氨酸特异性蛋白酶（Kgp）在放线放线杆菌生物膜的分离中是否发挥不同的作用。这项研究的目的是调查Kgp和Rgp在放线放线杆菌聚集和附着中可能的破坏作用。牙龈卟啉单胞菌ATCC33277培养物上清液具有降低放线放线杆菌细胞的自动聚集和共聚集的能力，而ATCC33277的煮沸的培养物上清液和KDP136的培养物上清液均未显示出该能力。分别加入Rgp和Kgp的特异性抑制剂KYT-1和KYT-36，对牙龈卟啉单胞菌培养物上清液的能力没有影响。明胶酶谱分析的结果表明，由牙龈蛋白酶处理的其他蛋白酶介导了放线杆菌的聚集减少。我们还通过评估A.放线菌从聚苯乙烯表面的分离，检查了牙龈蛋白酶的生物膜破坏作用。扫描电子显微镜分析表明，放线放线杆菌细胞被牙龈卟啉单胞菌Kgp分离。与牙龈卟啉单胞菌共培养时，生物膜中放线菌的数量减少。但是，添加KYT-36后未发现。这些发现表明，Kgp是放线放线杆菌生物膜分离和减少的关键成分。

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