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首页> 外文期刊>Molecular ecology resources >Starting a DNA barcode reference library for shallow water polychaetes from the southern European Atlantic coast
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Starting a DNA barcode reference library for shallow water polychaetes from the southern European Atlantic coast

机译：启动来自欧洲南部大西洋沿岸浅水多毛鱼类的DNA条形码参考文库

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Annelid polychaetes have been seldom the focus of dedicated DNA barcoding studies, despite their ecological relevance and often dominance, particularly in soft-bottom estuarine and coastal marine ecosystems. Here, we report the first assessment of the performance of DNA barcodes in the discrimination of shallow water polychaete species from the southern European Atlantic coast, focusing on specimens collected in estuaries and coastal ecosystems of Portugal. We analysed cytochrome oxidase I DNA barcodes (COI-5P) from 164 specimens, which were assigned to 51 morphospecies. To our data set from Portugal, we added available published sequences selected from the same species, genus or family, to inspect for taxonomic congruence among studies and collection location. The final data set comprised 290 specimens and 79 morphospecies, which generated 99 Barcode Index Numbers (BINs) within Barcode of Life Data Systems (BOLD). Among these, 22 BINs were singletons, 47 other BINs were concordant, confirming the initial identification based on morphological characters, and 30 were discordant, most of which consisted on multiple BINs found for the same morphospecies. Some of the most prominent cases in the latter category include Hediste diversicolor (O. F. Muller, 1776) (7), Eulalia viridis (Linnaeus, 1767) (2) and Owenia fusiformis (delle Chiaje, 1844) (5), all of them reported from Portugal and frequently used in ecological studies as environmental quality indicators. Our results for these species showed discordance between molecular lineages and morphospecies, or added additional relatively divergent lineages. The potential inaccuracies in environmental assessments, where underpinning polychaete species diversity is poorly resolved or clarified, demand additional and extensive investigation of the DNA barcode diversity in this group, in parallel with alpha taxonomy efforts.
机译：尽管阿尼利德多毛cha具有生态相关性并且经常占主导地位，但很少在专门的DNA条码研究中成为重点，特别是在软底河口和沿海海洋生态系统中。在这里，我们报告DNA条形码在区分来自南欧大西洋沿岸的浅水多毛鱼类物种中的性能的首次评估，重点是在葡萄牙的河口和沿海生态系统中收集的标本。我们分析了来自164个标本的细胞色素氧化酶I DNA条形码（COI-5P），这些标本被分配给51个形态物种。在我们来自葡萄牙的数据集中，我们添加了从相同物种，属或科中选择的可用公开序列，以检查研究和收集地点之间的分类学一致性。最终数据集包括290个标本和79个形态种，它们在生命条形码系统（BOLD）中生成了99个条形码索引号（BIN）。其中，22个BIN是单例的，其他47个BIN是一致的，证实了基于形态学特征的初步鉴定，还有30个是不协调的，其中大多数是由针对相同形态物种发现的多个BIN组成。在后一类中最突出的案例包括杂色Hediste（OF Muller，1776）（7），Eulalia viridis（Linnaeus，1767）（2）和Owenia fusiformis（delle Chiaje，1844）（5），所有这些都已报道。来自葡萄牙，经常在生态研究中用作环境质量指标。我们对这些物种的研究结果表明分子谱系和形态物种之间不一致，或者添加了其他相对发散的谱系。在环境评估中潜在的不准确之处，即解决多壳鱼类物种多样性的基础尚未得到很好的解决或弄清，因此需要在该组中对DNA条码多样性进行更多且广泛的研究，同时还要进行α分类法研究。

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