Green tea inhibits human inducible nitric-oxide synthase expression by down-regulating signal transducer and activator of transcription-1alpha activation.

Tedeschi E; Menegazzi M; Yao Y; Suzuki H; Forstermann U; Kleinert H

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Green tea inhibits human inducible nitric-oxide synthase expression by down-regulating signal transducer and activator of transcription-1alpha activation.

机译：绿茶通过下调信号转导子和转录1α激活因子来抑制人诱导型一氧化氮合酶的表达。

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Green tea has been reported to show anti-inflammatory properties because of its inhibitory effects on the expression of several pro-inflammatory genes. Because the inducible nitricoxide synthase (iNOS) plays an important role in chronic inflammatory diseases, we have focused our attention on the regulation of iNOS expression by green tea in two different human epithelial cell lines, alveolar A549/8 and colon DLD-1 cells. With the use of electrophoretic mobility shift assays, we found a green tea-mediated down-regulation of the DNA binding activity of the transcription factor signal transducer and activator of transcription-1alpha (STAT-1alpha), but not of nuclear factor-kappaB. This down-regulation of the STAT-1alpha DNA binding was shown to result from reduced tyrosine phosphorylation of the STAT-1alpha protein and not from antioxidative effects of the green tea extract. Green tea extract inhibited human iNOS expression in a concentration-dependent manner, quantified in terms of iNOS mRNA, iNOS protein, and nitric oxide production in both cell lines. This inhibitory effect of green tea resulted from transcriptional inhibition as shown in reporter gene experiments. These data suggest that green tea extracts may be promising at least as an auxiliary anti-inflammatory principle in chronic inflammatory diseases.

机译：据报道，由于绿茶对几种促炎基因的表达具有抑制作用，因此具有抗炎特性。由于诱导型一氧化氮合酶（iNOS）在慢性炎性疾病中起着重要作用，因此我们将注意力集中在绿茶对iNOS表达的调节上，这两种不同的人上皮细胞系是肺泡A549 / 8和结肠DLD-1细胞。通过使用电泳迁移率迁移分析，我们发现了绿茶介导的转录因子信号转导子和转录因子1alpha（STAT-1alpha）而不是核因子kappaB的DNA结合活性的下调。 STAT-1alpha DNA结合的这种下调显示是由于STAT-1alpha蛋白的酪氨酸磷酸化降低，而不是绿茶提取物的抗氧化作用。绿茶提取物以浓度依赖性的方式抑制人iNOS的表达，在两种细胞系中均以iNOS mRNA，iNOS蛋白和一氧化氮的产生进行定量。如报告基因实验所示，这种绿茶的抑制作用是由转录抑制引起的。这些数据表明，绿茶提取物至少有望作为一种慢性炎症性疾病的辅助抗炎原理。

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《Molecular pharmacology.》 |2004年第1期|共10页
作者
Tedeschi E; Menegazzi M; Yao Y; Suzuki H; Forstermann U; Kleinert H;
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正文语种 eng
中图分类药学;
关键词
Gene Expression Regulation; Enzymologic; NF-kappa B; Nitric-Oxide Synthase; 基因表达调控; 酶学; NF-κB; 一氧化氮合酶; 茶; 反式激活(遗传学);

机译：Gene Expression Regulation;Enzymologic;NF-kappa B;Nitric-Oxide Synthase;基因表达调控;酶学;NF-κB;一氧化氮合酶;茶;反式激活(遗传学);

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专利

1. Green tea inhibits human inducible nitric-oxide synthase expression by down-regulating signal transducer and activator of transcription-1alpha activation. [J] . Tedeschi E, Menegazzi M, Yao Y, Molecular pharmacology. . 2004,第1期

机译：绿茶通过下调信号转导子和转录1α激活因子来抑制人诱导型一氧化氮合酶的表达。
2. Orazipone inhibits activation of inflammatory transcription factors nuclear factor-kappa B and signal transducer and activator of transcription 1 and decreases inducible nitric-oxide synthase expression and nitric oxide production in response to infl [J] . Sareila O, Hamalainen M, Nissinen E, The Journal of Pharmacology and Experimental Therapeutics: Official Publication of the American Society for Pharmacology and Experimental Therapeutics . 2008,第2期

机译：Orazipone抑制炎症转录因子核因子-κB和信号转导和转录激活因子1的活化，并降低可诱导的一氧化氮合酶表达和一氧化氮的产生
3. Regulation of Lipopolysaccharide-lnduced Inducible Nitric-Oxide Synthase Expression through the Nuclear Factor-kappa B Pathway and lnterferon-beta/Tyrosine Kinase 2/Janus Tyrosine Kinase 2-Signal Transducer and Activator of Transcription-1 Signaling [J] . Hye Jung Kim, Konstantin Tsoyi, Ja Myung Heo The Journal of Pharmacology and Experimental Therapeutics: Official Publication of the American Society for Pharmacology and Experimental Therapeutics . 2007,第2期

机译：调节脂多糖诱导的一氧化氮合酶表达通过核因子-κB通路和干扰素-β/酪氨酸激酶2 / Janus酪氨酸激酶2-信号转导和转录1信号激活剂。
4. The Downregulation of Mast Cell Activation Through the Suppression of the High-Affinity IgE Receptor Expression by Green Tea Catechin Egcg [C] . Hirofumi Tachibana, Yoshinori Fujimura, Yusuke Hasegawa, Annual and International Meeting of the Japanese Association for Animal Cell Technology . 2010

机译：通过抑制绿茶儿茶素EGCG抑制高亲和力IgE受体表达的肥大细胞活化的下调
5. Protection against angiotensin II-induced endothelial dysfunction and hypertension via small molecule inhibitors of signal transducer and activator of transcription 3. [D] . Johnson, Andrew William. 2012

机译：通过信号转导和转录激活的小分子抑制剂保护血管紧张素II引起的内皮功能障碍和高血压3。
6. Heparin Cofactor II a Serine Protease Inhibitor Promotes Angiogenesis via Activation of the AMP-activated Protein Kinase-Endothelial Nitric-oxide Synthase Signaling Pathway [O] . Yasumasa Ikeda, Ken-ichi Aihara, Sumiko Yoshida, 2012

机译：肝素辅酶II一种丝氨酸蛋白酶抑制剂通过激活AMP激活的蛋白激酶-内皮型一氧化氮合酶信号通路来促进血管生成。
7. GH Inhibits Interferon- -Induced Signal Transducer and Activator of Transcription-1 Activation and Expression of the Inducible Isoform of Nitric Oxide Synthase in INS-1 Cells [O] . N. Sekine 2001

机译：GH抑制干扰素诱导的信号传感器和转录-1活化的活化剂 - 1个细胞中一氧化氮合酶的诱导型同种型的表达

Green tea inhibits human inducible nitric-oxide synthase expression by down-regulating signal transducer and activator of transcription-1alpha activation.

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