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Molecular phylogeny of Aerides (Orchidaceae) based on one nuclear and two plastid markers: A step forward in understanding the evolution of the Aeridinae

机译:基于一个核和两个质体标记的Aerides(兰科)的分子系统发育:向前了解Aeridinae进化的一步。

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Phylogenetic relationships of the orchid genus Aerides (Epidendroideae, Vandeae, Aeridinae) from Southeast Asia were inferred from DNA sequences of one nuclear (nrITS) and two plastid (matK, trnL-trnL-F) regions of 48 taxa (21 Aerides, 25 other Acridinae, 2 outgroup). Analyses of the combined datasets with parsimony, maximum likelihood and Bayesian methods revealed that Aerides is monophyletic and consists of three well-supported subclades which are only partly in accordance with previous sectional delimitations based on floral characters. The two different flower types in Aerides (hidden versus open spur entrance) seem to have evolved at least twice in geographically distinct areas. The phylogeny presented here is yet another example in Orchidaceae where floral morphology cannot be relied on to reconstruct phylogenetic history but rather is the result of pollinator-driven selection. The Aerides subclades are characterized by three different length classes of the mutation-rich P8 region in the trnL intron. To our knowledge, this is the first time that the P8 region was studied in orchids. The matK gene has been assumed to be a pseudogene in orchids due to occasional occurrence of frameshift indels, low transition/transversion (ts:tv) ratios and low substitution rates at the 3rd codon position. However, matK does not appear to be a pseudogene in Aerides and a comparison with data from other angiosperms suggests that ts:tv ratios and low substitution rates have been overestimated as arguments for a pseudogene status of matK in orchids. (c) 2008 Elsevier Inc. All rights reserved.
机译:从东南亚的兰花属植物Aerides(Epidendroideae,Vandeae,Aeridinae)的亲缘关系从48个分类单元(21个Aerides,25个其他)的一个核(nrITS)和两个质体(matK,trnL-trnL-F)区的DNA序列推论rid科(2)。用简约,最大似然和贝叶斯方法对组合数据集进行分析后发现,Aerides是单生的,由三个支持良好的子包组成,这些子包仅部分符合先前基于花卉特征的部分定界。 Aerides中的两种不同花型(隐藏的和开放的马刺入口)似乎在地理上不同的区域至少进化了两次。这里介绍的系统发育是兰科的另一个例子,其中不能依靠花的形态来重建系统发育史,而是传粉媒介驱动选择的结果。 Aerides子小节的特征是trnL内含子中富含突变的P8区的三种不同长度类别。据我们所知,这是首次在兰花中研究P8区域。由于偶尔出现移码插入缺失,低的过渡/转化(ts:tv)比和在第3个密码子位置的低取代率,matK基因被认为是兰花中的假基因。然而,在Aerides中matK似乎不是假基因,与其他被子植物的数据进行比较表明,ts:tv比和低取代率被高估了作为兰花matK假基因状态的理由。 (c)2008 Elsevier Inc.保留所有权利。

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