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首页> 外文期刊>Molecular phylogenetics and evolution >Molecular phylogenetics of tsetse flies (Diptera: Glossinidae) based on mitochondrial (COI, 16S, ND2) and nuclear ribosomal DNA sequences, with an emphasis on the palpalis group
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Molecular phylogenetics of tsetse flies (Diptera: Glossinidae) based on mitochondrial (COI, 16S, ND2) and nuclear ribosomal DNA sequences, with an emphasis on the palpalis group

机译：采采蝇（双翅目：Glossinidae）的分子系统发育基于线粒体（COI，16S，ND2）和核糖体DNA序列，重点是with骨

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摘要

Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In Particular, cytochrome oxidose 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented. (C) 2008 Elsevier Inc. All rights reserved.
机译：从线粒体（细胞色素氧化酶1，NADH脱氢酶2和16S）和核（rDNA的内部转录间隔区1）序列推断出Glossina属（采采蝇）的13种关系。所形成的系统发育学证实形态上限定的fusca，morsitans和palpalis亚属的单系。 pa舌和morsitans亚种之间的遗传距离表明它们的地位需要修改。特别是，细胞色素氧化糖1序列显示了G. palpalis palpalis内的巨大地理差异，表明该亚种内存在隐性物种。检查了pa骨组女性生殖器板的形态，发现个体在标准识别码指定的范围之外变化，从而无法进行明确的形态分类。提出了一种基于内部转录间隔区1序列的长度差异来区分G. palpalis papalalis，G。tachinoides和G. palpalis gambiensis的诊断PCR。 （C）2008 Elsevier Inc.保留所有权利。

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