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首页> 外文期刊>Molecules >Antioxidant and Antiproliferative Activities of Methanolic Extract from a Neglected Agricultural Product: Corn Cobs
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Antioxidant and Antiproliferative Activities of Methanolic Extract from a Neglected Agricultural Product: Corn Cobs

机译：来自被忽视的农产品：玉米芯的甲醇提取物的抗氧化和抗增殖活性

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摘要

Neglected agricultural products (NAPs) are defined as discarded material in agricultural production. Corn cobs are a major waste of agriculture maize. Here, a methanolic extract from corn cobs (MEC) was obtained. MEC contains phenolic compounds, protein, carbohydrates (1.4:0.001:0.001). We evaluated the in vitro and in vivo antioxidant potential of MEC. Furthermore, its antiproliferative property against tumor cells was assessed through MTT assays and proteins related to apoptosis in tumor cells were examined by western blot. MEC showed no hydroxyl radical scavenger capacity, but it showed antioxidant activity in Total Antioxidant Capacity and DPPH scavenger ability assays. MEC showed higher Reducing Power than ascorbic acid and exhibited high Superoxide Scavenging activity. In tumor cell culture, MEC increased catalase, metallothionein and superoxide dismutase expression in accordance with the antioxidant tests. In vivo antioxidant test, MEC restored SOD and CAT, decreased malondialdehyde activities and showed high Trolox Equivalent Antioxidant Capacity in animals treated with CCl4. Furthermore, MEC decreased HeLa cells viability by apoptosis due an increase of Bax/Bcl-2 ratio, caspase 3 active. Protein kinase C expression increased was also detected in treated tumor cells. Thus, our findings pointed out the biotechnological potential of corn cobs as a source of molecules with pharmacological activity.
机译：被忽视的农产品被定义为农业生产中的废弃材料。玉米芯是农业玉米的主要浪费。在此，获得了来自玉米芯（MEC）的甲醇提取物。 MEC包含酚类化合物，蛋白质，碳水化合物（1.4：0.001：0.001）。我们评估了MEC的体外和体内抗氧化潜力。此外，通过MTT分析评估了其对肿瘤细胞的抗增殖特性，并通过蛋白质印迹法检测了与肿瘤细胞凋亡相关的蛋白质。 MEC没有显示出羟基自由基清除剂的能力，但在总抗氧化剂能力和DPPH清除剂能力的测定中却显示出抗氧化剂的活性。与抗坏血酸相比，MEC的还原能力更高，并且具有较高的超氧化物清除活性。根据抗氧化剂测试，在肿瘤细胞培养中，MEC增加了过氧化氢酶，金属硫蛋白和超氧化物歧化酶的表达。在体内抗氧化剂测试中，MEC在用CCl4处理的动物中恢复了SOD和CAT，降低了丙二醛活性，并显示出高的Trolox等效抗氧化能力。此外，MEC通过增加Bax / Bcl-2比，激活caspase 3活性而通过凋亡降低HeLa细胞的生存能力。在治疗的肿瘤细胞中也检测到蛋白激酶C表达增加。因此，我们的发现指出了玉米芯作为具有药理活性的分子来源的生物技术潜力。

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