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The Relationships between Phenolic Content, Pollen Diversity, Physicochemical Information and Radical Scavenging Activity in Honey

机译：蜂蜜中酚类含量，花粉多样性，理化信息与自由基清除活性的关系

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摘要

Honey is rich in different secondary plant metabolites acting as natural antioxidants and contributing to human health. Radical scavenging activity (RSA) is related to antioxidant activity, while the correlation between the phenolic content and RSA is often weak. Consequently, exclusive information on phenolics is often insufficient to qualify the RSA and the health promoting effects of honey. The paper deals with a case study of honey samples originating from the alpine areas of Italy's Lombardia and Veneto regions and realized by standard physicochemical and statistical analytical methods. In pure honey, the total phenolic content and the RSA were measured in spectrophotometric tests with the 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free radical and Folin-Ciocalteu assays, respectively. Melissopalynological data was used to qualify pollen diversity through rank-frequency curves separating the samples into two groups. On the basis of physicochemical data, the samples were analyzed through multivariate classification and ranking procedures resulting in the identification of an outlier. Elimination of the outlier produced a high correlation between the total phenolic content and RSA in the two pollen diversity groups. The case study suggests that, after disregarding outliers, the RSA activity can be satisfactorily qualified on the basis of phenolics with pollen diversity as a covariate.
机译：蜂蜜富含作为自然抗氧化剂的多种次生植物代谢产物，有助于人体健康。自由基清除活性（RSA）与抗氧化剂活性相关，而酚含量与RSA之间的相关性通常较弱。因此，有关酚类的独家信息通常不足以使RSA和蜂蜜的健康促进作用合格。本文以来自意大利伦巴第大区和威尼托大区的高山地区的蜂蜜样品为例，并通过标准的理化和统计分析方法来实现。在纯蜂蜜中，总酚含量和RSA分别通过分光光度法分别通过2,2-二苯基-1-吡啶并肼基（DPPH·）自由基和Folin-Ciocalteu分析进行测定。通过将样本分成两组的等级-频率曲线，将褪灰粉学数据用于鉴定花粉多样性。根据理化数据，通过多变量分类和排序程序对样本进行分析，从而确定异常值。消除异常值在两个花粉多样性组中产生了总酚含量与RSA之间的高度相关性。案例研究表明，在忽略异常值之后，可以基于以花粉多样性为协变量的酚类化合物令人满意地鉴定RSA活性。

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