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首页> 外文期刊>Mutation Research - Genetic Toxicology and Environmental Mutagenesis >Cyto-genotoxic effects of smoke from commercial filter and non-filter cigarettes on human bronchial and pulmonary cells
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Cyto-genotoxic effects of smoke from commercial filter and non-filter cigarettes on human bronchial and pulmonary cells

机译：商用滤嘴香烟和非滤嘴香烟烟雾对人支气管和肺细胞的细胞遗传毒性作用

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摘要

Cigarette smoke is a complex mixture of chemicals, some of which are known as carcinogens. The cyto-genotoxic effects of cigarette-smoke extract (CSE) from commercial cigarettes without (A and B) and with filter (C and D) were evaluated at different CSE concentrations on A549 and BEAS-2B cells. The particle content of the cigarette smoke and the metal composition of the CSE were also analyzed. The cells were exposed to 1-10% of the CSE from one cigarette per experiment. Cytotoxicity was evaluated by use of the MTT assay after 24. h, and the lactate dehydrogenase (LDH) assay after 30. min and 24. h. The Fpg-modified comet assay was used to evaluate direct-oxidative DNA damage on cells exposed for 30. min. As expected, unfiltered cigarette smoke (particularly from the B cigarette) contained a higher number of particles than filtered smoke. With smoke extract from the B cigarette we found a decrease in cell viability only in BEAS-2B cells. The results of the LDH test showed membrane damage for B-cigarette smoke extract, particularly in BEAS-2B cells. Extracts from unfiltered cigarette smoke induced significant direct DNA damage, to a larger extent in A549 cells. Filtered cigarette-smoke extract induced a significant direct DNA damage at 5-10%. A significant induction of oxidative DNA damage was found at the highest CSE concentration in both cell types (by smoke extracts from B and C cigarettes in A549 cells, and from A and D cigarettes in BEAS-2B cells). Smoke extracts from filter cigarettes induced less direct DNA damage than those from unfiltered cigarettes in A549 cells, probably due to a protective effect of filter. In BEAS-2B cells the smoke extract from the B-cigarette showed the highest genotoxic effect, with a concentration-dependent trend.These findings show a higher cyto-genotoxicity for smoke extracts from the B-cigarette and oxidative effects for those from the A and D cigarettes, particularly in BEAS-2B cells. Moreover, there was a higher responsiveness of A549 cells to genotoxic insult of CSE, and a cigarette-dependent genotoxicity in BEAS-2B cells. Our experimental model demonstrated to be suitable to sensitively detect early genotoxic response of different lung-cell types to non-cytotoxic concentrations of complex inhalable mixtures.
机译：香烟烟雾是化学物质的复杂混合物，其中一些被称为致癌物。在不同的CSE浓度下，对A549和BEAS-2B细胞在不同浓度的CSE情况下，评估了不含（A和B）和带滤嘴（C和D）的商用卷烟的烟熏提取物（CSE）的细胞遗传毒性作用。还分析了香烟烟雾的颗粒含量和CSE的金属成分。每个实验中，这些细胞从一根香烟中暴露于1-10％的CSE。在24. h后使用MTT分析，并在30. min和24. h后使用乳酸脱氢酶（LDH）分析来评估细胞毒性。使用Fpg修饰的彗星试验评估暴露30分钟的细胞的直接氧化DNA损伤。不出所料，未过滤的香烟烟雾（尤其是B香烟）比过滤的烟雾含有更多的颗粒。使用B支香烟的烟雾提取物，我们发现仅在BEAS-2B细胞中细胞活力下降。 LDH测试的结果显示B香烟烟雾提取物的膜受损，特别是在BEAS-2B细胞中。未经过滤的香烟烟雾中的提取物在A549细胞中引起了很大的直接DNA损伤。过滤后的烟熏提取物可导致5-10％的直接DNA明显损伤。在两种细胞类型中，最高的CSE浓度下都发现了明显的氧化DNA损伤诱导作用（通过A549细胞的B和C香烟和BEAS-2B细胞的A和D香烟的烟雾提取物）。与未过滤香烟相比，来自过滤嘴香烟的烟雾提取物对A549细胞的直接DNA损伤要少。在BEAS-2B细胞中，B烟的烟提取物表现出最高的遗传毒性作用，且呈浓度依赖性趋势，这些发现表明B烟的烟提取物具有更高的细胞遗传毒性，而A烟的提取物具有氧化作用。和D型香烟，特别是在BEAS-2B细胞中。此外，A549细胞对CSE的遗传毒性损害具有更高的响应性，而BEAS-2B细胞则具有香烟依赖的遗传毒性。我们的实验模型证明适合灵敏地检测不同肺细胞类型对复杂可吸入混合物的非细胞毒性浓度的早期遗传毒性反应。

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