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首页> 外文期刊>Mutation Research, A. Reviews in Genetic Toxicology >Genotoxicity assessment of aromatic amines and amides in genetically engineered V79 cells
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Genotoxicity assessment of aromatic amines and amides in genetically engineered V79 cells

机译：基因工程V79细胞中芳香胺和酰胺的遗传毒性评估

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摘要

A genetically engineered V79 cell line expressing rat CVP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyllransferase activity, as well as CYP-deficient parental V79 cell tines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminoiluorene, 4-acetylaminofluorrene and 2-arnino-3-niethylimidazo[4,5-f]quinolinc, with chromosomal aberrations and sister chro malid exchanges as the end-points. None of the test compounds showed a clear effect on the [requency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorcnc and 2-acetylaminofluorene in the GYPlA2-profieient cells, but not in the CYPlA2-deficicnl cells. The presence of acctyltransferase activity enhanced the effect of 2-aminoanthracenc, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-krnino-3-methyliniidazo[4,5-f]quinolinc did not induce sister chromatidexchanges in the investigated cell lines. The use of cell lines with defined mclabolic capabilities seems to be a valuable tool to study specific metabolic pathway's important in the activation of procarcinogens.
机译：使用经过基因工程改造的表达大鼠CVP1A2的V79细胞系和表达大鼠CYP1A2以及内源性乙酰转移酶活性的另一种细胞系以及CYP缺陷型亲代V79细胞株，来评估芳香胺和酰胺2-氨基蒽的遗传毒性， 2-氨基芴，2-乙酰氨基芴，4-乙酰氨基芴和2-氨基-3-镍基咪唑并[4,5-f]喹啉，以染色体畸变和姊妹色氨酸苹果酸交换为终点。在所有使用的细胞系中，没有一种测试化合物对染色体畸变的频率有明显的影响。但是，姊妹染色单体交换是由GYPIA2细胞中的2-氨基蒽，2-氨基芴和2-乙酰氨基芴诱导的，而在CYP1A2-缺陷细胞中则没有。酰基转移酶活性的存在增强了2-氨基蒽，2-氨基芴和2-乙酰基氨基芴的作用。 4-乙酰氨基芴和2-krinino-3-methyliniidazo [4,5-f] quinolinc不会在研究的细胞系中诱导姐妹染色素变化。具有确定的代谢功能的细胞系的使用似乎是研究特定代谢途径在致癌物激活中重要的有价值的工具。

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