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首页> 外文期刊>Mutation Research, A. Reviews in Genetic Toxicology >A comparison of the 8-hydroxydeoxyguanosine, chromosome aberrations and' micronucleus techniques for the assessment of the genotoxicity of mercury compounds in human blood lymphocytes
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A comparison of the 8-hydroxydeoxyguanosine, chromosome aberrations and' micronucleus techniques for the assessment of the genotoxicity of mercury compounds in human blood lymphocytes

机译：比较8-羟基脱氧鸟苷，染色体畸变和微核技术评估人血淋巴细胞中汞化合物的遗传毒性

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We compared the mechanism of action of microriuclei (MN), unstable chromosome aberrations, and 8-hydroxyde-oxyguanosine (8-OHdG) levels to evaluate the genotoxicity of methyl mercuric chloride (CH3HgCl) and mercuric chloride (HgCl_2) in human peripheral lymphocytes. The chromosome aberrations in human peripheral lymphocytes exposed to various concentrations of CH_3HgCl or HgCl_2 increased in a concentration-dependent manner and were significantly higher than the control when the cells were incubatedwith 1 X 10~(-5) M (HgCl_2) or 2 X 10~(-6) M (CH3HgCl). The increase in the incidence of micronucleated lymphocytes was significant among the exposed groups, being 2 X 10~(-5) M (HgCl_2) and 5 X 10~(-6) M (CH_3HgGl) compared with the control, CH_3HgCI was about 4-fold more potent man HgCl_2. We determined the 8-OHdG levels in human peripheral blood mononuclear cells(PBMC) and found that they were significantly higher in the exposed groups at 1 X 10~s M (HgCl2) and 5 X 10~(-6) M (CH_3HgCl) compared withthe control, A detectable(p < 0.05) increase in the level of 8-OHdG was induced by CH_3HgCl at a concentration that was about 50% of the amount of HgCl_2 required to produce a similar response. The data confirmed the value of the MN and/or chromosome aberration assays for assessing of HgCl_2- and/ or CH_3HgCWnduced genotoxicity, and indicated that they are about the same concentration as the 8-OHdG assay. The presence of genotoxic effects in peripheral blood lymphocytes exposed to the mercuric compoundsindicated by the chromosome aberrations and the MN assays could be partly due either to the disturbance of the spindle mechanism, or to the elevated level of 8-OHdG brought by the generation of reactive oxygen species.
机译：我们比较了微核仁（MN），不稳定的染色体畸变和8-羟基脱氧鸟苷（8-OHdG）水平的作用机理，以评估甲基汞氯化物（CH3HgCl）和氯化汞（HgCl_2）在人外周淋巴细胞中的遗传毒性。暴露于不同浓度的CH_3HgCl或HgCl_2的人外周血淋巴细胞的染色体畸变以浓度依赖的方式增加，并且在将细胞与1 X 10〜（-5）M（HgCl_2）或2 X 10孵育时显着高于对照组。 〜（-6）M（CH3HgCl）。在暴露组中，微核淋巴细胞的发生率显着增加，与对照相比，CH_3HgCl约为2 X 10〜（-5）M（HgCl_2）和5 X 10〜（-6）M（CH_3HgGl）。 HgCl_2的效价倍数。我们测定了人外周血单个核细胞（PBMC）中的8-OHdG水平，发现它们在暴露组中显着更高，分别为1 X 10〜s M（HgCl2）和5 X 10〜（-6）M（CH_3HgCl）与对照相比，CH_3HgCl诱导的8-OHdG水平可检测到的（p <0.05）升高，浓度约为产生类似反应所需HgCl_2量的50％。数据证实了MN和/或染色体畸变分析对评估HgCl_2和/或CH_3HgCW引起的基因毒性的价值，并表明它们的浓度与8-OHdG分析大致相同。在暴露于汞化合物的外周血淋巴细胞中，由染色体畸变和MN检测表明存在遗传毒性作用，部分原因可能是纺锤体机制受到干扰，或者是由于反应性物质的产生导致了8-OHdG水平升高氧气种类。

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