• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mutation Research, C. Mutation Research Letters >VARIABILITY IN ADAPTIVE RESPONSE TO LOW DOSE RADIATION IN HUMAN BLOOD LYMPHOCYTES - CONSISTENT RESULTS FROM CHROMOSOME ABERRATIONS AND MICRONUCLEI
【24h】

VARIABILITY IN ADAPTIVE RESPONSE TO LOW DOSE RADIATION IN HUMAN BLOOD LYMPHOCYTES - CONSISTENT RESULTS FROM CHROMOSOME ABERRATIONS AND MICRONUCLEI

机译：人体淋巴细胞对低剂量辐射的适应性反应的变异性-染色体畸变和微核的一致结果

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The frequencies of chromosome aberrations and micronuclei were evaluated to assess the induction of adaptive response to low dose ionizing radiation in each of the blood samples collected from eight different individuals. Following stimulation with phytohemagglutinin, the cells were exposed to an adaptive dose of 1 cGy X-radiation at 24 hours and a challenge dose of 150 cGy gamma radiation at 48 hours. Lymphocytes were fixed at 54 hours to examine the incidence of chromosome aberrations and at 72 hours to examine the frequency of micronuclei in cytokinesis-blocked binucleated cells. Lymphocytes from five donors, i.e., ''responders'', exhibited the induction of adaptive response; their lymphocytes, which were pre-treated with 1 cGy had significantly fewer chromosome aberrations and micronuclei induced by the challenge dose of 150 coy gamma radiation, as compared to the cells which did not receive the pre-treatment with 1 cGy. Such an induction of adaptive response was not observed in the remaining three donors, i.e., ''non-responders''; the incidence of chromosome aberrations and micronuclei induced by the challenge dose of 150 cGy was not significantly different between the cells which were pre-exposed and un-exposed to 1 cGy. In all eight individuals, there was a strong positive correlation between the incidence of chromosome aberrations and micronuclei. Hence, whether or not an individual is a 'responder' or 'non-responder' could be assessed using either chromosome aberrations or micronuclei as the end-point. The overall pattern of response confirms the heterogeneity in adaptive response between individuals to ionizing radiation, which may in part be genetically controlled. Because of the simplicity of the technique and rapid assessment of the binucleated cells, we suggest the use of the micronucleus test as an alternative procedure in large scale population studies related to the adaptive response.
机译：评估了染色体畸变和微核的频率，以评估从八个不同个体收集的每个血液样本中对低剂量电离辐射的适应性反应的诱导。用植物血凝素刺激后，细胞在24小时暴露于适应剂量的1 cGy X射线，在48小时暴露于激发剂量150 cGyγ射线。将淋巴细胞固定在54小时以检查染色体畸变的发生率，并在72小时以固定在胞质分裂阻滞的双核细胞中微核的频率。来自五个供体的淋巴细胞，即“应答者”，表现出适应性应答的诱导。与未接受1 cGy预处理的细胞相比，用1 cGy预处理的淋巴细胞与未接受1 cGy预处理的细胞相比，受到150 coyγ射线激发剂量诱导的染色体畸变和微核明显更少。在其余三个捐助者，即“无反应者”中未观察到这种适应性反应的诱导; 150 cGy激发剂量诱导的染色体畸变和微核的发生率在预先暴露和未暴露于1 cGy的细胞之间没有显着差异。在所有八个个体中，染色体畸变的发生率与微核之间有很强的正相关。因此，可以使用染色体畸变或微核作为终点来评估一个人是“响应者”还是“非响应者”。总体反应模式证实了个体之间对电离辐射的适应性反应的异质性，这在某种程度上可以通过基因控制。由于技术的简单性和对双核细胞的快速评估，我们建议在与适应性反应相关的大规模人群研究中，使用微核试验作为替代程序。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号