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首页> 外文期刊>Mutation Research, C. Mutation Research Letters >MICRONUCLEUS ASSAY IN PULMONARY ALVEOLAR MACROPHAGES, A SIMPLE MODEL TO DETECT GENOTOXICITY OF ENVIRONMENTAL AGENTS ENTERING THROUGH THE INHALATION ROUTE
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MICRONUCLEUS ASSAY IN PULMONARY ALVEOLAR MACROPHAGES, A SIMPLE MODEL TO DETECT GENOTOXICITY OF ENVIRONMENTAL AGENTS ENTERING THROUGH THE INHALATION ROUTE

机译：肺泡巨噬细胞中的微核试验，一种检测通过吸入途径进入环境的个体的遗传毒性的简单模型

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摘要

A simple and short-term micronucleus (MN) test in pulmonary alveolar macrophages (PAMs) of rats has been developed to assess potential genotoxic effects of gaseous environmental agents. The protocol has been tested in model experiments with indoor air pollutants like mosquito coil smoke (MCS) and mosquito mat vapour (MMV). Smears of pulmonary lavage fluid collected in hypotonic (0.56%) KCl solution were fixed in absolute methanol and stained in Giemsa (10%). Characteristically the large size of the PAMs facilitates easy scoring of MN. An interval of 32 h post exposure seems to be suitable for MN preparation. A comparison of the concentration-response data on CAs (at 24 h post exposure) and MN (at 32 h post exposure) clearly reveals the validity of the MN assay in PAMs.
机译：已经开发了一种简单且短期的大鼠肺泡巨噬细胞（PAM）微核（MN）测试，以评估气态环境药物的潜在遗传毒性作用。该协议已在模型实验中使用室内空气污染物（例如，蚊香（MCS）和蚊帐蒸气（MMV））进行了测试。在低渗（0.56％）KCl溶液中收集的肺灌洗液涂片在无水甲醇中固定，并在Giemsa（10％）中染色。典型地，PAM的大尺寸便于对MN进行评分。暴露后32小时的间隔似乎适合于MN的制备。对CAs（暴露后24小时）和MN（暴露后32小时）的浓度-响应数据的比较清楚地揭示了MN测定在PAM中的有效性。

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