A COMPARATIVE STUDY OF TK6 HUMAN LYMPHOBLASTOID AND L5178Y MOUSE LYMPHOMA CELL LINES IN THE IN VITRO MICRONUCLEUS TEST

Zhang LS; Honma M; Hayashi M; Suzuki T; Matsuoka A; Sofuni T

首页> 外文期刊>Mutation Research, C. Mutation Research Letters >A COMPARATIVE STUDY OF TK6 HUMAN LYMPHOBLASTOID AND L5178Y MOUSE LYMPHOMA CELL LINES IN THE IN VITRO MICRONUCLEUS TEST

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A COMPARATIVE STUDY OF TK6 HUMAN LYMPHOBLASTOID AND L5178Y MOUSE LYMPHOMA CELL LINES IN THE IN VITRO MICRONUCLEUS TEST

机译：TK6人淋巴母细胞和L5178Y小鼠淋巴瘤细胞系体外微核试验的比较研究

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Micronucleus induction was compared in human lymphoblastoid TK6 and mouse lymphoma L5178Y cell lines treated with model clastogens and spindle poisons, i.e., X-rays, methyl methanesulfonate, ethyl methanesulfonate, mitomycin C, colcemid, and vincristine. The spontaneous micronucleated cell (MNC) frequency was stable and reproducible in both cell lines. All clastogens and spindle poisons studied here induced micronuclei in both cell lines. They increased MNC frequency at lower concentrations or caused a greater increase at the same concentration in TK6 cells. These clastogens and spindle poisons, however, were also more toxic to TK6 than to L5178Y cells and when comparison was based on cytotoxicity, they showed more efficient MNC induction in L5178Y cells. In conclusion, neither cell line was superior to the other, and both of them can be used as target cells in the in vitro micronucleus assay.

机译：比较了用模型分裂原和梭形毒物（即X射线，甲磺酸甲酯，甲磺酸乙酯，丝裂霉素C，秋水仙碱和长春新碱）处理的人淋巴母细胞TK6和小鼠淋巴瘤L5178Y细胞系中的微核诱导。在两种细胞系中，自发性微核细胞（MNC）的频率均稳定且可重现。此处研究的所有酶原和纺锤体毒物均可诱导两种细胞系中的微核。它们在较低浓度下增加了MNC频率，或者在TK6细胞中在相同浓度下引起了更大的增加。然而，这些克拉斯蛋白酶原和纺锤体毒物对TK6的毒性也比对L5178Y细胞的毒性高，当基于细胞毒性进行比较时，它们在L5178Y细胞中的MNC诱导效率更高。总之，两种细胞系都不比另一种细胞优越，并且它们都可以用作体外微核试验中的靶细胞。

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《Mutation Research, C. Mutation Research Letters》 |1995年第4期|共11页
作者
Zhang LS; Honma M; Hayashi M; Suzuki T; Matsuoka A; Sofuni T;
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正文语种 eng
中图分类遗传学;
关键词
In vitro micronucleus test; Human lymphoblastoid cells; Tk6; Mouse; Cytokinesis block method; Hamster lung cells; Chromosome damage; Cytochalasin b; Assay; Frequency; Invitro; Lymphocytes; Aberrations; Survival;

机译：体外微核试验;人类淋巴母细胞;Tk6;小鼠;细胞分裂阻滞法;仓鼠肺细胞;染色体损伤;细胞松弛素b;测定;频率;体外;淋巴细胞;畸变;生存;

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专利

1. A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test. [J] . Zhang LS, Honma M, Hayashi M, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1995,第3a4期

机译：TK6人淋巴母细胞和L5178Y小鼠淋巴瘤细胞系体外微核试验的比较研究。
2. A COMPARATIVE STUDY OF TK6 HUMAN LYMPHOBLASTOID AND L5178Y MOUSE LYMPHOMA CELL LINES IN THE IN VITRO MICRONUCLEUS TEST [J] . Zhang LS, Honma M, Hayashi M, Mutation Research, C. Mutation Research Letters . 1995,第3a4期

机译：TK6人淋巴母细胞和L5178Y小鼠淋巴瘤细胞系体外微核试验的比较研究
3. Flow-cytometric analysis of in vitro micronucleus formation: Comparative studies with WIL2-NS human lymphoblastoid and L5178Y mouse lymphoma cell lines. [J] . Laingam S, Froscio SM, Humpage AR Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 2008,第1a2期

机译：体外微核形成的流式细胞术分析：与WIL2-NS人淋巴母细胞样细胞和L5178Y小鼠淋巴瘤细胞系的比较研究。
4. The effects benzo(a)pyrenediolexpoxide exposure on DNA adduct formation, altered gene expression, and toxicity in human lymphoblastoid TK6 cells. [C] . Joshua J Klaene, Jennifer Barrett, Caroline Ceailles Flakaros, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：苯并（a）Pyrenyiolexpatiene暴露在DNA加合物形成，改变基因表达和人淋巴细胞Tk6细胞中的效果暴露。
5. Arsenite alters lysosome-mediated degradation and the autophagy process leading to immunosuppression in human B-lymphoblastoid cell lines. [D] . Bolt, Alicia Marie. 2012

机译：亚砷酸盐可改变溶酶体介导的降解和自噬过程，从而导致人B淋巴母细胞细胞系中的免疫抑制。
6. In vitro genotoxicity studies: n-Butyl acrylate L5178Y mouse lymphoma (TK+/− locus assay) 2-Ethylhexyl acrylate gene mutation assay in Chinese hamster V79 cells and 2-Ethylhexyl acrylate micronucleus test in human lymphocytes [O] . Sandra Murphy, Robert Ellis-Hutchings, Lavorgie Finch, 2018

机译：体外遗传毒性研究：丙烯酸正丁酯L5178Y小鼠淋巴瘤（TK +/-基因座测定）中国仓鼠V79细胞中的丙烯酸2-乙基己酯基因突变测定和人体淋巴细胞中丙烯酸2-乙基己酯微核试验
7. A combination of in vitro comet assay and micronucleus test using human lymphoblastoid TK6 cells [O] . A. Kimura, A. Miyata, M. Honma 2013

机译：使用人淋巴细胞TK6细胞的体外彗星测定和微核试验的组合
8. Evaulation of the Mutagenic Activity of T5870.3 in an In Vitro Mammalian Cell Gene Mutation Test with L5178Y Mouse Lymphoma Cells (with Independent Repeat) [R] . 2003

机译：用L5178Y小鼠淋巴瘤细胞（具有独立重复）进行体外哺乳动物细胞基因突变试验中T5870.3致突变活性的推断

A COMPARATIVE STUDY OF TK6 HUMAN LYMPHOBLASTOID AND L5178Y MOUSE LYMPHOMA CELL LINES IN THE IN VITRO MICRONUCLEUS TEST

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