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首页> 外文期刊>Molecular Breeding >Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato
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Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato

机译：番茄中Ty-1和Ty-3基因赋予的番茄抗黄叶卷曲病毒基因型选择

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摘要

The tomato yellow leaf curl virus (TYLCV), transmitted by whitefly, causes major disease losses to tomato crops in tropical and subtropical regions of the world. Several genes conferring resistance to TYLCV, mainly Ty-1 and Ty-3 genes, have been introgressed to cultivated tomato (Solanum lycopersicum) from the wild relative species Solanum chilense. By combining bulked segregant analysis and amplified fragment length polymorphisms (AFLP), several AFLP markers closely linked to Ty-1 and Ty-3 genes were identified from the resistant breeding line TZ841-4. Cloning and sequencing of the selected AFLP fragments allowed us to develop codominant cleaved amplified polymorphic sequence and dominant sequence characterized amplified region markers closely linked to Ty-1. In addition, Ty-3-linked allelic-specific markers have been discriminated by a quantitative real-time PCR protocol. Taken together, these markers constitute useful tools for marker-assisted selection breeding programs to improve genetic resistance to TYLCV, and also to initiate map-based cloning approaches to isolate the resistance genes.
机译：烟粉虱传播的番茄黄叶卷曲病毒（TYLCV）导致世界热带和亚热带地区的番茄作物遭受重大疾病损失。几种赋予TYLCV抗性的基因，主要是Ty-1和Ty-3基因，已被引入野生近缘种茄茄的栽培番茄（番茄）。通过结合大量的分离子分析和扩增的片段长度多态性（AFLP），从抗性育种系TZ841-4中鉴定了与Ty-1和Ty-3基因紧密相关的几个AFLP标记。所选AFLP片段的克隆和测序使我们能够开发共切的扩增多态性序列和表征与Ty-1紧密相连的扩增区标记的显性序列。另外，已经通过定量实时PCR方案区分了Ty-3连接的等位基因特异性标记。这些标记共同构成了标记辅助选择育种程序的有用工具，以提高对TYLCV的遗传抗性，并启动基于图谱的克隆方法以分离抗性基因。

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