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首页> 外文期刊>Molecular Breeding >Phenotypic gain from introgression of two QTL, qSB9-2 and qSB12-1, for rice sheath blight resistance
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Phenotypic gain from introgression of two QTL, qSB9-2 and qSB12-1, for rice sheath blight resistance

机译：从两个QTL基因渗入获得的表型增益，分别针对水稻皮枯萎病

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摘要

F2:3 families from crosses between three rice indica introgression lines and their common japonica recurrent parent were used to evaluate two quantitative trait loci (QTL) for sheath blight (SB) resistance. Three selected TeQing-into-Lemont backcross introgression lines (TILs) were more resistant than their susceptible parent (Lemont) in inoculated field plots, and were molecularly verified to contain TeQing alleles at qSB9-2 and/or qSB12-1. F2 individuals homozygous for qSB9-2 and qSB12-1 provided F2:3 families that fit four genotypic classes: containing the resistant TeQing allele for qSB9-2 (TQ) alone, qSB12-1 (TQ) alone, both qSB9-2 (TQ) and qSB12-1 (TQ) , and neither SB QTL introgression. By comparing the SB resistance of these four genotypic classes in micro-chamber evaluations and inoculated field plots, the phenotypic values of the QTL were measured. Under both study conditions, disease resistance ranked qSB9-2 + qSB12-1 > qSB9-2 > qSB12-1 > no QTL, with both qSB9-2 and qSB12-1 acting as dominant resistance genes. In micro-chamber studies, qSB9-2 (TQ) reduced disease an average of 1.0 disease index units and qSB12-1 (TQ) by 0.7 using a scale of 0-9. Field effects of qSB9-2 (TQ) and qB12-1 (TQ) were less pronounced, with average phenotypic gains of 0.5 and 0.2 units, respectively. TIL:642 proved to contain qSB9-2 (TQ) in an introgression so small it was tagged by just RM205 on the tip of chromosome 9. These studies verify that the indica introgression of qSB9-2 (TQ) or qSB12-1 (TQ) can measurably improve resistance to sheath blight disease in a highly susceptible tropical japonica cultivar, and fine-mapped the qSB9-2 locus. Markers presently verified as linked to these QTL can support marker-assisted breeding to improve disease resistance.
机译：利用三个three稻基因渗入系与普通粳稻轮回亲本的杂交后代的F2：3家族评估两个定量性状基因座（QTL）对白叶枯病（SB）的抗性。在接种的田间地块中，三个选定的TeQing-into-Lemont回交渗入系（TILs）比其易感亲本（Lemont）更具抗性，并且经过分子验证，它们在qSB9-2和/或qSB12-1处包含TeQing等位基因。对qSB9-2和qSB12-1纯合的F2个体提供了适合四个基因型类别的F2：3家族：包含仅针对qSB9-2（TQ），仅针对qSB12-1（TQ）的抗性TeQing等位基因，以及针对qSB9-2（TQ ）和qSB12-1（TQ），并且SB QTL都没有渗入。通过在微腔室评估和接种场图中比较这四种基因型类别的SB抗性，可以测量QTL的表型值。在这两种研究条件下，抗病性等级分别为qSB9-2 + qSB12-1> qSB9-2> qSB12-1>无QTL，其中qSB9-2和qSB12-1均为优势抗性基因。在微腔室研究中，qSB9-2（TQ）使用0-9的等级将疾病平均减少1.0个疾病指数单位，qSB12-1（TQ）减少0.7。 qSB9-2（TQ）和qB12-1（TQ）的场效应不太明显，平均表型增益分别为0.5和0.2个单位。 TIL：642证明在渗入体中含有qSB9-2（TQ）如此之小，仅在9号染色体尖端被RM205标记。这些研究证实了qSB9-2（TQ）或qSB12-1（TQ）的in体渗入）可以显着提高高度敏感的热带粳稻品种对枯萎病的抗性，并精细定位qSB9-2基因座。目前证实与这些QTL相关的标记可以支持标记辅助育种，以提高抗病性。

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