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首页> 外文期刊>Mutation research. Genetic toxicology testing >A MINIATURIZED AMES MUTAGENICITY ASSAY EMPLOYING BIOLUMINESCENT STRAINS OF SALMONELLA TYPHIMURIUM
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A MINIATURIZED AMES MUTAGENICITY ASSAY EMPLOYING BIOLUMINESCENT STRAINS OF SALMONELLA TYPHIMURIUM

机译：应用鼠伤寒沙门氏菌生物发光菌株的最小化AMES突变检测

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Increased awareness of the role of environmental factors in carcinogenesis has led to an emphasis on preventing or minimizing exposure to genotoxicants. This is presently promoting the development of simple, rapid, cost-effective mutagenicity screening assays. We have developed a test system based on the well-known Salmonella mutagenicity assay. The lux genes, which permit cells to emit light through bioluminescence, were introduced into Salmonella typhimurium strain TA98. These bacteria were exposed for 48 h to chemicals or complex mixtures in 48-well microplates containing an appropriate liquid medium. Cells were subsequently centrifuged and resuspended in buffer, The final postexposure revertant biomass was then estimated using a microluminometer, Replication trials confirmed methodological reproducibility. Clear dose-response relationships were obtained with the direct frameshift mutagens 4NQO and 2NF. Mutagenicity threshold effect concentrations found for these compounds were comparable to those reported in the literature. Industrial effluents and environmental extracts (effluents, suspended solids) were also tested and results compared well with those of the SOS Chromotest. While further validation of this new adaptation of the Ames test will be required, it appears at this time that it could be well suited for routine screening of xenobiotics and environmental samples.
机译：人们越来越认识到环境因素在致癌作用中的作用，导致人们着重于预防或尽量减少暴露于遗传毒性物质。目前，这促进了简单，快速，具有成本效益的诱变性筛选测定法的发展。我们已经基于著名的沙门氏菌诱变性测定法开发了一种测试系统。将允许细胞通过生物发光发光的勒克斯基因引入鼠伤寒沙门氏菌菌株TA98中。这些细菌在含有适当液体培养基的48孔微孔板中暴露于化学品或复杂混合物48小时。随后将细胞离心并重悬于缓冲液中，然后使用微光度计估算最终的暴露后回复生物量，复制试验证实了方法的可重复性。使用直接移码诱变剂4NQO和2NF获得了明确的剂量反应关系。这些化合物的致突变性阈值效应浓度与文献报道的相当。还测试了工业废水和环境提取物（废水，悬浮固体），并将结果与​​SOS Chromotest进行了比较。虽然将需要对Ames试验的这种新适应性进行进一步验证，但目前看来它很适合常规筛选异种生物和环境样品。

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