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首页> 外文期刊>Molecular Breeding >Enhanced salinity stress tolerance in transgenic chilli pepper (Capsicum annuum L.) plants overexpressing the wheat antiporter (TaNHX2) gene
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Enhanced salinity stress tolerance in transgenic chilli pepper (Capsicum annuum L.) plants overexpressing the wheat antiporter (TaNHX2) gene

机译：过表达小麦反转运蛋白（TaNHX2）基因的转基因辣椒植物中的盐分胁迫耐受性增强

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摘要

Transgenic chilli pepper (Capsicum annuum L.) plants tolerant to salinity stress were produced by introducing the wheat Na+/H+? antiporter gene (TaNHX2) via Agrobacterium-mediated transformation. Cotyledonary explants were infected with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pBin438 that contains a wheat antiporter (TaNHX2) gene driven by the double CaMV 35S promoter and NPT II gene as a selectable marker. PCR and semiquantitative RT-PCR analysis confirmed that the TaNHX2 gene had been integrated and expressed in the T-1 generation of transgenic pepper plants as compared to the non-transformed plants. Southern blot analysis further verified the integration and presence of TaNHX2 gene in the genome of chilli pepper plants. Biochemical assays of these transgenic plants revealed enhanced levels of proline, chlorophyll, superoxide dismutase, ascorbate peroxidase, relative water content, and reduced levels of hydrogen peroxide (H2O2), malondialdehyde compared to wild-type plants under salt stress conditions. The present investigation clearly showed that overexpression of the TaNHX2 gene enhanced salt stress tolerance in transgenic chilli pepper plants.
机译：通过引入小麦Na + / H + 2可以生产耐盐胁迫的转基因辣椒植物（Capsicum annuum L.）。通过农杆菌介导的转化的反转运蛋白基因（TaNHX2）。子叶外植体被根癌农杆菌菌株LBA4404感染，该菌株带有二元载体pBin438，该载体包含由双重CaMV 35S启动子驱动的小麦反转运蛋白（TaNHX2）基因和NPT II基因作为选择标记。 PCR和半定量RT-PCR分析证实，与未转化的植物相比，TaNHX2基因已经整合并在T-1代转基因辣椒植物中表达。 Southern印迹分析进一步证实了TaNHX2基因在辣椒植物基因组中的整合和存在。这些转基因植物的生化分析表明，与野生型植物相比，在盐胁迫条件下，脯氨酸，叶绿素，超氧化物歧化酶，抗坏血酸过氧化物酶，相对水含量和过氧化氢（H2O2），丙二醛含量降低。本研究清楚地表明，TaNHX2基因的过表达增强了转基因辣椒植物的盐胁迫耐受性。

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