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Use of doubled haploids in maize breeding: implications for intellectual property protection and genetic diversity in hybrid crops

机译:双倍单倍体在玉米育种中的应用:对杂交作物知识产权保护和遗传多样性的影响

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摘要

Through the concerted use of doubled haploidy (DH), molecular markers and off-season nurseries, maize (Zea mays L.) breeders have unprecedented capabilities to quickly and precisely create progeny with desired levels of similarity to either parents of a commercial hybrid. Genotypic data from both simulated and from actual populations created either by single seed descent or through doubled haploidy were examined for the initial and subsequent generations. Simulation data showed that DH progeny inherited larger blocks of parental chromosomes; approximately seven out of 10 chromosomes had intact segments of 50% or greater. By the third DH generation progeny can be selected that are more than 90% similar to either parent of the initial commercial hybrid. Actual marker data from the initial DH generation showed a maximum parental contribution of 88.4% compared to 78.7% for progeny developed by single seed descent (SSD). The number of intact chromosomes was higher among DH progeny than among progeny bred by SSD. Use of DH facilitates access to germplasm that is already present in commercial maize hybrids. Available technologies coupled with the intellectual property protection regime will influence decisions made by plant breeders in the balance of exotic compared to well-adapted germplasm they choose to access for further cycles.
机译:通过双倍单倍体(DH),分子标记和淡季育苗室的共同使用,玉米(Zea mays L.)育种者具有空前的能力,可以快速,精确地创建与商业杂交双亲的期望水平相似的后代。通过模拟和实际种群的基因型数据,无论是单种子后代还是双倍单倍体,都可以检查它们的初代和后代。模拟数据表明,DH后代遗传了较大的亲本染色体块; 10条染色体中大约有7条的完整片段为50%或更高。到第三DH代,可以选择与最初的商业杂种的任一亲本相似度超过90%的后代。最初的DH代的实际标记数据显示,最大的父母亲贡献为88.4%,而通过单种子后代(SSD)发育的后代则为78.7%。 DH后代中完整染色体的数量比SSD繁殖后代中的完整染色体数量高。 DH的使用促进了商业玉米杂交种中已经存在的种质的获取。现有技术与知识产权保护制度的结合,将影响植物育种者在异国情调与他们选择进入进一步循环的适应性种质之间取得平衡的决定。

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